• Western blot analysis of extracts of 293 and HepG2 cells, using Phospho-INSR-Y1150/IGF1R-Y1135 antibody (STJ22192) at 1:1000 dilution. 293 cells were treated by EGF (25ug/mL) for 30 minutes after serum-starvation overnight. HepG2 cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
  • Western blot analysis of extracts of 293 and HepG2 cells, using Phospho-INSR-Y1150/IGF1R-Y1135 antibody (STJ22192) at 1:1000 dilution. 293 cells were treated by EGF (25 Mu g/mL) for 30 minutes after serum-starvation overnight. HepG2 cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA.

Anti-Phospho-IGF1R/INSR-Y1150/Y1135 antibody (STJ22192)

SKU:
STJ22192

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-IGF1R/INSR-Y1150/Y1135 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: A synthetic phosphorylated peptide around Y1150 of human IGF1RINSR (NP_001073285.1).
Immunogen Sequence: TDYYR
Background The protein encoded by this gene is a protease that deubiquitinates target proteins such as ADORA2A and TRIM21. The encoded protein shuttles between the nucleus and cytoplasm and is involved in maintaining operational fidelity in the endoplasmic reticulum. Three transcript variants encoding different isoforms have been found for this gene.

Information sourced from Uniprot.org

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