Anti-Phospho-IFNGR1-Tyr457 antibody (431-480 aa) (STJ91086)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human Interferon-gamma Receptor alpha around the phosphorylation site of Tyr457 at the amino acid range 431-480
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Interferon gamma receptor 1-Tyr457 (431-480 aa) for use in WB, IHC, IF and ELISA in Human, Mouse, Rat and Monkey samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat/Monkey |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | IFNGR1 |
| Gene ID | 3459 |
| Uniprot ID | INGR1_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human Interferon-gamma Receptor alpha around the phosphorylation site of Tyr457 at the amino acid range 431-480 |
| Immunogen Region | 431-480 aa |
| Specificity | Phospho-IFN-Gamma R Alpha (Y457) Polyclonal Antibody detects endogenous levels of IFN-Gamma R Alpha protein only when phosphorylated at Y457. |
Additional Info
| Function | Receptor subunit for interferon gamma/INFG that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation. Associates with transmembrane accessory factor IFNGR2 to form a functional receptor. Upon ligand binding, the intracellular domain of IFNGR1 opens out to allow association of downstream signaling components JAK1 and JAK2. In turn, activated JAK1 phosphorylates IFNGR1 to form a docking site for STAT1. Subsequent phosphorylation of STAT1 leads to dimerization, translocation to the nucleus, and stimulation of target gene transcription. STAT3 can also be activated in a similar manner although activation seems weaker. IFNGR1 intracellular domain phosphorylation also provides a docking site for SOCS1 that regulates the JAK-STAT pathway by competing with STAT1 binding to IFNGR1. |
| Protein Name | Interferon Gamma Receptor 1Ifn-Gamma Receptor 1Ifn-Gamma-R1Cdw119Interferon Gamma Receptor Alpha-ChainIfn-Gamma-R-AlphaCd Antigen Cd119 |
| Database Links | Reactome: R-HSA-877300Reactome: R-HSA-877312Reactome: R-HSA-9679191Reactome: R-HSA-9732724 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-Interferon Gamma Receptor 1 antibodyAnti-Ifn-Gamma Receptor 1 antibodyAnti-Ifn-Gamma-R1 antibodyAnti-Cdw119 antibodyAnti-Interferon Gamma Receptor Alpha-Chain antibodyAnti-Ifn-Gamma-R-Alpha antibodyAnti-Cd Antigen Cd119 antibodyAnti-IFNGR1 antibody |
Information sourced from Uniprot.org