• Western blot analysis of extracts of NIH/3T3 cells, using Phospho-Histone H3. 3-T3 rabbit monoclonal antibody (STJ11102645) at 1:1000 dilution. NIH/3T3 cells were treated by Nocodazole (50 ng/mL) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 3min.
  • Western blot analysis of lysates from C6 cells, using Phospho-Histone H3. 3-T3 Rabbit monoclonal antibody (STJ11102645) at 1:1000 dilution. C6 cells were treated by Nocodazole (50 ng/mL) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Western blot analysis of extracts of HeLa cells, using Phospho-Histone H3. 3-T3 rabbit monoclonal antibody (STJ11102645) at 1:1000 dilution. HeLa cells were treated by nocodazole (50 ng/mL) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of lysates from HeLa cells, using Phospho-Histone H3. 3-T3 Rabbit monoclonal antibody (STJ11102645) at 1:1000 dilution. HeLa cells were treated by nocodazole (50 ng/mL) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts of C6 cells, using Phospho-Histone H3. 3-T3 rabbit monoclonal antibody (STJ11102645) at 1:1000 dilution. C6 cells were treated by Nocodazole (50 ng/mL) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Histone H3. 3-T3 Rabbit monoclonal antibody (STJ11102645) at 1:1000 dilution. NIH/3T3 cells were treated by Nocodazole (50 ng/mL) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-Phospho-Histone H3-T3 antibody [S5MR] (STJ11102645)

SKU:
STJ11102645

$197.78 - $867.38
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Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat/Other
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: H3C1.H3C2.H3C3.H3C4.H3C6.H3C7.H3C8.H3C10.H3C11.H3C12
8350/8351/8352/8353/8354/8355/8356/8357/8358/8968
8290
Uniprot ID: H31_HUMAN
H31T_HUMAN
Specificity: A synthetic phosphorylated peptide around T3 of human Histone H3 (P84243).
Immunogen Sequence: MARTK
Tissue Specificity P68431 - NA
Q16695 - Expressed in testicular cells.
Alternative Antibody Names Anti-Histone H3.1 antibody
Anti-Histone H3/A antibody
Anti-Histone H3/B antibody
Anti-Histone H3/C antibody
Anti-Histone H3/D antibody
Anti-Histone H3/F antibody
Anti-Histone H3/H antibody
Anti-Histone H3/I antibody
Anti-Histone H3/J antibody
Anti-Histone H3/K antibody
Anti-Histone H3/L antibody
Anti-H3C1 antibody
Anti-H3FA antibody
Anti-HIST1H3A.H3C2 antibody
Anti-H3FL antibody
Anti-HIST1H3B.H3C3 antibody
Anti-H3FC antibody
Anti-HIST1H3C.H3C4 antibody
Anti-H3FB antibody
Anti-HIST1H3D.H3C6 antibody
Anti-H3FD antibody
Anti-HIST1H3E.H3C7 antibody
Anti-H3FI antibody
Anti-HIST1H3F.H3C8 antibody
Anti-H3FH antibody
Anti-HIST1H3G.H3C10 antibody
Anti-H3FK antibody
Anti-HIST1H3H.H3C11 antibody
Anti-H3FF antibody
Anti-HIST1H3I.H3C12 antibody
Anti-H3FJ antibody
Anti-HIST1H3J antibody
Anti-Histone H3.1t antibody
Anti-H3/T antibody
Anti-H3t antibody
Anti-H3/G antibody
Anti-Histone H3.4 antibody
Anti-H3-4 antibody
Anti-H3FT antibody
Anti-HIST3H3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance