• Western blot analysis of lysates from A549 cells treated with PMA 125ng/ml 30', using HBP1 (Phospho-Ser402) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Western blot analysis of A549 cells using Phospho-HBP1 (S402) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-HBP1-Ser402 antibody (371-420 aa) (STJ90526)

SKU:
STJ90526

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-HMG box-containing protein 1-Ser402 (371-420 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: HBP1
Gene ID: 26959
Uniprot ID: HBP1_HUMAN
Immunogen Region: 371-420 aa
Specificity: Phospho-HBP1 (S402) Polyclonal Antibody detects endogenous levels of HBP1 protein only when phosphorylated at S402.
Immunogen: The antiserum was produced against synthesized peptide derived from the human HBP1 around the phosphorylation site of Ser402 at the amino acid range 371-420
Function Transcriptional repressor that binds to the promoter region of target genes. Plays a role in the regulation of the cell cycle and of the Wnt pathway. Binds preferentially to the sequence 5'-TTCATTCATTCA-3'. Binding to the histone H1.0 promoter is enhanced by interaction with RB1. Disrupts the interaction between DNA and TCF4.
Protein Name Hmg Box-Containing Protein 1
Hmg Box Transcription Factor 1
High Mobility Group Box Transcription Factor 1
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Hmg Box-Containing Protein 1 antibody
Anti-Hmg Box Transcription Factor 1 antibody
Anti-High Mobility Group Box Transcription Factor 1 antibody
Anti-HBP1 antibody

Information sourced from Uniprot.org

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