• Western blot analysis of extracts of various cell lines, using Phospho-GYS1-S641 antibody (STJ11101015) at 1:1000 dilution. NIH/3T3 cells and C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of lysates from 293T cells, using Phospho-GYS1-S641 polyclonal antibody (STJ11101015) at 1:1000 dilution or Glycogen synthase 1 (GYS1) antibody (A2519). 293T cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of extracts of 293T cells, using Phospho-GYS1-S641 polyclonal antibody (STJ11101015) at 1:1000 dilution or Glycogen synthase 1 (GYS1) antibody (A2519). 293T cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of various lysates using Phospho-GYS1-S641 Rabbit polyclonal antibody (STJ11101015) at 1:1000 dilution. NIH/3T3 cells and C6 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Phospho-GYS1-S641 antibody (STJ11101015)

SKU:
STJ11101015

£212.50 - £501.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-GYS1-S641 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GYS1
Gene ID: 2997
Uniprot ID: GYS1_HUMAN
Immunogen: A synthetic phosphorylated peptide around S641 of human Glycogen synthase 1 (GYS1) (NP_002094.2).
Immunogen Sequence: PASVP
Post Translational Modifications Phosphorylation at Ser-8 by AMPK inactivates the enzyme activity. Primed phosphorylation at Ser-657 (site 5) by CSNK2A1 and CSNK2A2 is required for inhibitory phosphorylation at Ser-641 (site 3a), Ser-645 (site 3b), Ser-649 (site 3c) and Ser-653 (site 4) by GSK3A an GSK3B. Phosphorylated at Ser-641 by DYRK2, leading to inactivation. Phosphorylated at Ser-641 by PASK, leading to inactivation.phosphorylation by PASK is inhibited by glycogen. Dephosphorylation at Ser-641 and Ser-645 by PP1 activates the enzyme.
Function Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.
Protein Name Glycogen Starch Synthase - Muscle
Database Links Reactome: R-HSA-3322077
Reactome: R-HSA-3785653
Reactome: R-HSA-3814836
Reactome: R-HSA-3828062
Alternative Antibody Names Anti-Glycogen Starch Synthase - Muscle antibody
Anti-GYS1 antibody
Anti-GYS antibody

Information sourced from Uniprot.org

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