• Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 30', using GRK2 (Phospho-Ser29) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using GRK2 (Phospho-Ser29) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-GRK2-Ser29 antibody (14-63 aa) (STJ90813)

SKU:
STJ90813

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Beta-adrenergic receptor kinase 1-Ser29 (14-63 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GRK2
Gene ID: 156
Uniprot ID: ARBK1_HUMAN
Immunogen Region: 14-63 aa
Specificity: Phospho-GRK 2 (S29) Polyclonal Antibody detects endogenous levels of GRK 2 protein only when phosphorylated at S29.
Immunogen: The antiserum was produced against synthesized peptide derived from the human GRK2 around the phosphorylation site of Ser29 at the amino acid range 14-63
Function Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner. Positively regulates ciliary smoothened (SMO)-dependent Hedgehog (Hh) signaling pathway by facilitating the trafficking of SMO into the cilium and the stimulation of SMO activity. Inhibits relaxation of airway smooth muscle in response to blue light.
Protein Name Beta-Adrenergic Receptor Kinase 1
Beta-Ark-1
G-Protein Coupled Receptor Kinase 2
Database Links Reactome: R-HSA-111933
Reactome: R-HSA-416476
Reactome: R-HSA-418555
Reactome: R-HSA-5635838
Reactome: R-HSA-8856825
Cellular Localisation Cytoplasm
Cell Membrane
Postsynapse
Presynapse
Alternative Antibody Names Anti-Beta-Adrenergic Receptor Kinase 1 antibody
Anti-Beta-Ark-1 antibody
Anti-G-Protein Coupled Receptor Kinase 2 antibody
Anti-GRK2 antibody
Anti-ADRBK1 antibody
Anti-BARK antibody
Anti-BARK1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance