• Western blot analysis of extracts of various cell lines, using Phospho-GRIN2B-Y1474 polyclonal antibody (STJ116391) at 1:1000 dilution. NIH/3T3 cells were treated by EGF (100 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of various lysates using Phospho-GRIN2B-Y1474 polyclonal antibody (STJ116391) at 1:1000 dilution. NIH/3T3 cells were treated by EGF (100 ng/mL) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Phospho-GRIN2B-Y1474 antibody (STJ116391)

SKU:
STJ116391

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-GRIN2B-Y1474 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GRIN2B
Gene ID: 2904
Uniprot ID: NMDE2_HUMAN
Immunogen: A synthetic phosphorylated peptide around Y1474 of human GRIN2B (NP_000825.2).
Immunogen Sequence: HVYEK
Tissue Specificity Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia.
Post Translational Modifications Phosphorylated on tyrosine residues. Phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity.
Function Component of NMDA receptor complexes that function as heterotetrameric, ligand-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Channel activation requires binding of the neurotransmitter glutamate to the epsilon subunit, glycine binding to the zeta subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+). Sensitivity to glutamate and channel kinetics depend on the subunit composition. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death. Contributes to neural pattern formation in the developing brain. Plays a role in long-term depression (LTD) of hippocampus membrane currents and in synaptic plasticity.
Protein Name Glutamate Receptor Ionotropic - Nmda 2b
Glun2b
Glutamate Nmda Receptor Subunit Epsilon-2
N-Methyl D-Aspartate Receptor Subtype 2b
Nmdar2b
Nr2b
N-Methyl-D-Aspartate Receptor Subunit 3
Nr3
Hnr3
Database Links Reactome: R-HSA-3928662
Reactome: R-HSA-438066
Reactome: R-HSA-442982
Reactome: R-HSA-5673001
Reactome: R-HSA-6794361
Reactome: R-HSA-8849932
Reactome: R-HSA-9022699
Reactome: R-HSA-9032500
Reactome: R-HSA-9609736
Reactome: R-HSA-9617324
Reactome: R-HSA-9620244
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Postsynaptic Cell Membrane
Late Endosome
Lysosome
Cytoplasm
Cytoskeleton
Co-Localizes With The Motor Protein Kif17 Along Microtubules
Alternative Antibody Names Anti-Glutamate Receptor Ionotropic - Nmda 2b antibody
Anti-Glun2b antibody
Anti-Glutamate Nmda Receptor Subunit Epsilon-2 antibody
Anti-N-Methyl D-Aspartate Receptor Subtype 2b antibody
Anti-Nmdar2b antibody
Anti-Nr2b antibody
Anti-N-Methyl-D-Aspartate Receptor Subunit 3 antibody
Anti-Nr3 antibody
Anti-Hnr3 antibody
Anti-GRIN2B antibody
Anti-NMDAR2B antibody

Information sourced from Uniprot.org

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