• Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-GJA1-Tyr265 antibody (STJ90609)

SKU:
STJ90609

Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Gap junction alpha-1 protein-Tyr265 is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GJA1
Gene ID: 2697
Uniprot ID: CXA1_HUMAN
Specificity: Phospho-Connexin 43 (S265) Polyclonal Antibody detects endogenous levels of Connexin 43 protein only when phosphorylated at S265.
Immunogen: Synthesized phospho-peptide around the phosphorylation site of human Connexin 43 (phospho Ser265)
Post Translational Modifications Phosphorylated at Ser-368 by PRKCG.phosphorylation induces disassembly of gap junction plaques and inhibition of gap junction activity. Phosphorylation at Ser-325, Ser-328 and Ser-330 by CK1 modulates gap junction assembly. Phosphorylation at Ser-368 by PRKCD triggers its internalization into small vesicles leading to proteasome-mediated degradation. Sumoylated with SUMO1, SUMO2 and SUMO3, which may regulate the level of functional Cx43 gap junctions at the plasma membrane. May be desumoylated by SENP1 or SENP2. S-nitrosylation at Cys-271 is enriched at the muscle endothelial gap junction in arteries, it augments channel permeability and may regulate of smooth muscle cell to endothelial cell communication. Acetylated in the developing cortex.leading to delocalization from the cell membrane.
Function Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract. May play a role in cell growth inhibition through the regulation of NOV expression and localization. Plays an essential role in gap junction communication in the ventricles.
Protein Name Gap Junction Alpha-1 Protein
Connexin-43
Cx43
Gap Junction 43 Kda Heart Protein
Database Links Reactome: R-HSA-190704
Reactome: R-HSA-190827
Reactome: R-HSA-190840
Reactome: R-HSA-190861
Reactome: R-HSA-190873
Reactome: R-HSA-191650
Reactome: R-HSA-196025
Reactome: R-HSA-9013406
Reactome: R-HSA-9013409
Reactome: R-HSA-9705677
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Cell Junction
Gap Junction
Endoplasmic Reticulum
Localizes At The Intercalated Disk (Icd) In Cardiomyocytes And The Proper Localization At Icd Is Dependent On Tmem65
Alternative Antibody Names Anti-Gap Junction Alpha-1 Protein antibody
Anti-Connexin-43 antibody
Anti-Cx43 antibody
Anti-Gap Junction 43 Kda Heart Protein antibody
Anti-GJA1 antibody
Anti-GJAL antibody

Information sourced from Uniprot.org

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