• Western blot analysis of extracts from K-562 cells, using Phospho-GATA1-S310 antibody (STJ22152). Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
  • Western blot analysis of extracts from K-562 cells, using Phospho-GATA1-S310 antibody (STJ22152). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA.

Anti-Phospho-GATA1-S310 antibody (STJ22152)

SKU:
STJ22152

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-GATA1-S310 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: GATA1
Gene ID: 2623
Uniprot ID: GATA1_HUMAN
Immunogen: A phospho specific peptide corresponding to residues surrounding S310 of human GATA1
Immunogen Sequence: Email for sequence
Tissue Specificity Erythrocytes.
Post Translational Modifications Highly phosphorylated on serine residues. Phosphorylation on Ser-310 is enhanced on erythroid differentiation. Phosphorylation on Ser-142 promotes sumoylation on Lys-137. Sumoylation on Lys-137 is enhanced by phosphorylation on Ser-142 and by interaction with PIAS4. Sumoylation with SUMO1 has no effect on transcriptional activity. Acetylated at 2 conserved lysine-rich motifs by CREBBP in vitro. Acetylation does not affect DNA-binding in vitro but is essential to induce erythroid differentiation and for binding chromatin in vivo. Acetylated on Lys-233, Lys-245 Lys-246 by EP300.
Function Transcriptional activator or repressor which serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-ATGATAAG-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS.
Protein Name Erythroid Transcription Factor
Eryf1
Gata-Binding Factor 1
Gata-1
Gf-1
Nf-E1 Dna-Binding Protein
Database Links Reactome: R-HSA-8936459
Reactome: R-HSA-8939236
Reactome: R-HSA-983231
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Erythroid Transcription Factor antibody
Anti-Eryf1 antibody
Anti-Gata-Binding Factor 1 antibody
Anti-Gata-1 antibody
Anti-Gf-1 antibody
Anti-Nf-E1 Dna-Binding Protein antibody
Anti-GATA1 antibody
Anti-ERYF1 antibody
Anti-GF1 antibody

Information sourced from Uniprot.org

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