• Western blot analysis of extracts from 293 cells, using Phospho-G3BP1-S232 antibody (STJ22148). Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
  • Western blot analysis of lysates from 293 cells, using Phospho-G3BP1-S232 Rabbit polyclonal antibody (STJ22148). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA.
  • Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-G3BP1-S232 antibody (STJ22148).
  • Immunofluorescence analysis of methanol-fixed HeLa cells using Phospho-G3BP1-S232 antibody (STJ22148).

Anti-Phospho-G3BP1-S232 antibody (STJ22148)

SKU:
STJ22148

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Host: Rabbit
Applications: WB/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-G3BP1-S232 is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:100-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: G3BP1
Gene ID: 10146
Uniprot ID: G3BP1_HUMAN
Immunogen: A phospho specific peptide corresponding to residues surrounding S232 of human Phospho-G3BP1-S232
Immunogen Sequence: Email for sequence
Tissue Specificity Ubiquitous.
Post Translational Modifications Phosphorylation of the acidic disordered region regulates stress granule assembly. RASA1-dependent phosphorylation of Ser-149 induces a conformational change that prevents self-association. Dephosphorylation after HRAS activation is required for stress granule assembly. Ser-149 phosphorylation induces partial nuclear localization. Ubiquitinated by TRIM21 via 'Lys-63'-linked polyubiquitination in the NTF2 domain in response to heat shock, leading to stress granule disassembly: ubiquitination promotes interaction with the FAF2 adapter, followed by interaction with VCP, which extracts G3BP1 from stress granules, leading to stress granule disassembly. In case of prolonged stress, ubiquitination by TRIM21 leads to autophagy-dependent degradation of G3BP1 via recruitment of ubiquitinated G3BP1 by SQSTM1 and/or CALCOCO2 to autophagosomes. (Microbial infection) Cleaved by human enterovirus 71.this cleavage induces the disassembly of cytoplasmic stress granules. Cleaved by Foot-and-mouth disease virus.this cleavage suppresses the formation of cytoplasmic stress granules. Arg-435 is dimethylated, probably to asymmetric dimethylarginine.
Function Protein involved in various processes, such as stress granule formation and innate immunity. Plays an essential role in stress granule formation. Stress granules are membraneless compartments that store mRNAs and proteins, such as stalled translation pre-initiation complexes, in response to stress. Promotes formation of stress granules phase-separated membraneless compartment by undergoing liquid-liquid phase separation (LLPS) upon unfolded RNA-binding: functions as a molecular switch that triggers RNA-dependent LLPS in response to a rise in intracellular free RNA concentrations. Also acts as an ATP- and magnesium-dependent helicase: unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Plays an essential role in innate immunity by promoting CGAS and RIGI activity. Participates in the DNA-triggered cGAS/STING pathway by promoting the DNA binding and activation of CGAS. Triggers the condensation of cGAS, a process probably linked to the formation of membrane-less organelles. Enhances also RIGI-induced type I interferon production probably by helping RIGI at sensing pathogenic RNA. May also act as a phosphorylation-dependent sequence-specific endoribonuclease in vitro: Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR.
Protein Name Ras Gtpase-Activating Protein-Binding Protein 1
G3bp-1
Atp-Dependent Dna Helicase Viii
Hdh Viii
Gap Sh3 Domain-Binding Protein 1
Database Links Reactome: R-HSA-9705671
Cellular Localisation Cytoplasm
Cytosol
Perikaryon
Stress Granule
Nucleus
Cytoplasmic In Proliferating Cells
Cytosolic And Partially Nuclear In Resting Cells
Recruited To Stress Granules In Response To Arsenite Treatment
The Unphosphorylated Form Is Recruited To Stress Granules
Hras Signaling Contributes To This Process By Regulating G3bp Dephosphorylation
Alternative Antibody Names Anti-Ras Gtpase-Activating Protein-Binding Protein 1 antibody
Anti-G3bp-1 antibody
Anti-Atp-Dependent Dna Helicase Viii antibody
Anti-Hdh Viii antibody
Anti-Gap Sh3 Domain-Binding Protein 1 antibody
Anti-G3BP1 antibody
Anti-G3BP antibody

Information sourced from Uniprot.org

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