Anti-Phospho-FOXM1-Thr600 antibody (STJ196307)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized phosho peptide around human FoxM1 (Thr600)
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Forkhead box protein M1-Thr600 for use in WB in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Dilution Range | WB 1:1000-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | FOXM1 |
| Gene ID | 2305 |
| Uniprot ID | FOXM1_HUMAN |
| Immunogen | Synthesized phosho peptide around human FoxM1 (Thr600) |
| Specificity | This antibody detects endogenous levels of Human FoxM1 (phospho-Thr600) |
Additional Info
| Post Translational Modifications | Phosphorylated in M (mitotic) phase. Phosphorylation by the checkpoint kinase CHEK2 in response to DNA damage increases the FOXM1 protein stability probably stimulating the transcription of genes involved in DNA repair. Phosphorylated by CDK1 in late S and G2 phases, creating docking sites for the POLO box domains of PLK1. Subsequently, PLK1 binds and phosphorylates FOXM1, leading to activation of transcriptional activity and subsequent enhanced expression of key mitotic regulators. Phosphorylated by GSK3B leading to ubiquitination and proteasomal degradation. Ubiquitinated in a FBXW7-dependent manner leading to proteasomal degradation. |
| Function | Transcription factor regulating the expression of cell cycle genes essential for DNA replication and mitosis. Plays a role in the control of cell proliferation. Also plays a role in DNA break repair, participating in the DNA damage checkpoint response. Promotes transcription of PHB2. |
| Protein Name | Forkhead Box Protein M1Forkhead-Related Protein Fkhl16Hepatocyte Nuclear Factor 3 Forkhead Homolog 11Hfh-11Hnf-3/Fork-Head Homolog 11M-Phase Phosphoprotein 2Mpm-2 Reactive Phosphoprotein 2Transcription Factor TridentWinged-Helix Factor From Ins-1 Cells |
| Database Links | Reactome: R-HSA-156711Reactome: R-HSA-69273Reactome: R-HSA-9725371 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Forkhead Box Protein M1 antibodyAnti-Forkhead-Related Protein Fkhl16 antibodyAnti-Hepatocyte Nuclear Factor 3 Forkhead Homolog 11 antibodyAnti-Hfh-11 antibodyAnti-Hnf-3/Fork-Head Homolog 11 antibodyAnti-M-Phase Phosphoprotein 2 antibodyAnti-Mpm-2 Reactive Phosphoprotein 2 antibodyAnti-Transcription Factor Trident antibodyAnti-Winged-Helix Factor From Ins-1 Cells antibodyAnti-FOXM1 antibodyAnti-FKHL16 antibodyAnti-HFH11 antibodyAnti-MPP2 antibodyAnti-WIN antibody |
Information sourced from Uniprot.org