• Western blot analysis of lysates from Jurkat cells treated with starved 24h, using FER (Phospho-Tyr402) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human brain, using FER (Phospho-Tyr402) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-FER-Tyr402 antibody (371-420 aa) (STJ90943)

SKU:
STJ90943

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Tyrosine-protein kinase Fer-Tyr402 (371-420 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: FER
Gene ID: 2241
Uniprot ID: FER_HUMAN
Immunogen Region: 371-420 aa
Specificity: Phospho-Fer (Y402) Polyclonal Antibody detects endogenous levels of Fer protein only when phosphorylated at Y402.
Immunogen: The antiserum was produced against synthesized peptide derived from the human FER around the phosphorylation site of Tyr402 at the amino acid range 371-420
Post Translational Modifications Autophosphorylated. Polyubiquitinated.this leads to proteasomal degradation.
Function Tyrosine-protein kinase that acts downstream of cell surface receptors for growth factors and plays a role in the regulation of the actin cytoskeleton, microtubule assembly, lamellipodia formation, cell adhesion, cell migration and chemotaxis. Acts downstream of EGFR, KIT, PDGFRA and PDGFRB. Acts downstream of EGFR to promote activation of NF-kappa-B and cell proliferation. May play a role in the regulation of the mitotic cell cycle. Plays a role in the insulin receptor signaling pathway and in activation of phosphatidylinositol 3-kinase. Acts downstream of the activated FCER1 receptor and plays a role in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Plays a role in the regulation of mast cell degranulation. Plays a role in leukocyte recruitment and diapedesis in response to bacterial lipopolysaccharide (LPS). Plays a role in synapse organization, trafficking of synaptic vesicles, the generation of excitatory postsynaptic currents and neuron-neuron synaptic transmission. Plays a role in neuronal cell death after brain damage. Phosphorylates CTTN, CTNND1, PTK2/FAK1, GAB1, PECAM1 and PTPN11. May phosphorylate JUP and PTPN1. Can phosphorylate STAT3, but the biological relevance of this depends on cell type and stimulus.
Protein Name Tyrosine-Protein Kinase Fer
Feline Encephalitis Virus-Related Kinase Fer
Fujinami Poultry Sarcoma/Feline Sarcoma-Related Protein Fer
Proto-Oncogene C-Fer
Tyrosine Kinase 3
P94-Fer
Database Links Reactome: R-HSA-1433557
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Cell Projection
Cell Junction
Membrane
Nucleus
Cell Cortex
Associated With The Chromatin
Detected On Microtubules In Polarized And Motile Vascular Endothelial Cells
Colocalizes With F-Actin At The Cell Cortex
Colocalizes With Pecam1 And Ctnnd1 At Nascent Cell-Cell Contacts
Alternative Antibody Names Anti-Tyrosine-Protein Kinase Fer antibody
Anti-Feline Encephalitis Virus-Related Kinase Fer antibody
Anti-Fujinami Poultry Sarcoma/Feline Sarcoma-Related Protein Fer antibody
Anti-Proto-Oncogene C-Fer antibody
Anti-Tyrosine Kinase 3 antibody
Anti-P94-Fer antibody
Anti-FER antibody
Anti-TYK3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance