| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-Phospho-Tyrosine-protein kinase Fer-Tyr402 (371-420 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:20000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | FER |
| Gene ID: | 2241 |
| Uniprot ID: | FER_HUMAN |
| Immunogen Region: | 371-420 aa |
| Specificity: | Phospho-Fer (Y402) Polyclonal Antibody detects endogenous levels of Fer protein only when phosphorylated at Y402. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human FER around the phosphorylation site of Tyr402 at the amino acid range 371-420 |
| Post Translational Modifications | Autophosphorylated. Polyubiquitinated.this leads to proteasomal degradation. |
| Function | Tyrosine-protein kinase that acts downstream of cell surface receptors for growth factors and plays a role in the regulation of the actin cytoskeleton, microtubule assembly, lamellipodia formation, cell adhesion, cell migration and chemotaxis. Acts downstream of EGFR, KIT, PDGFRA and PDGFRB. Acts downstream of EGFR to promote activation of NF-kappa-B and cell proliferation. May play a role in the regulation of the mitotic cell cycle. Plays a role in the insulin receptor signaling pathway and in activation of phosphatidylinositol 3-kinase. Acts downstream of the activated FCER1 receptor and plays a role in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Plays a role in the regulation of mast cell degranulation. Plays a role in leukocyte recruitment and diapedesis in response to bacterial lipopolysaccharide (LPS). Plays a role in synapse organization, trafficking of synaptic vesicles, the generation of excitatory postsynaptic currents and neuron-neuron synaptic transmission. Plays a role in neuronal cell death after brain damage. Phosphorylates CTTN, CTNND1, PTK2/FAK1, GAB1, PECAM1 and PTPN11. May phosphorylate JUP and PTPN1. Can phosphorylate STAT3, but the biological relevance of this depends on cell type and stimulus. |
| Protein Name | Tyrosine-Protein Kinase Fer Feline Encephalitis Virus-Related Kinase Fer Fujinami Poultry Sarcoma/Feline Sarcoma-Related Protein Fer Proto-Oncogene C-Fer Tyrosine Kinase 3 P94-Fer |
| Database Links | Reactome: R-HSA-1433557 |
| Cellular Localisation | Cytoplasm Cytoskeleton Cell Membrane Peripheral Membrane Protein Cytoplasmic Side Cell Projection Cell Junction Membrane Nucleus Cell Cortex Associated With The Chromatin Detected On Microtubules In Polarized And Motile Vascular Endothelial Cells Colocalizes With F-Actin At The Cell Cortex Colocalizes With Pecam1 And Ctnnd1 At Nascent Cell-Cell Contacts |
| Alternative Antibody Names | Anti-Tyrosine-Protein Kinase Fer antibody Anti-Feline Encephalitis Virus-Related Kinase Fer antibody Anti-Fujinami Poultry Sarcoma/Feline Sarcoma-Related Protein Fer antibody Anti-Proto-Oncogene C-Fer antibody Anti-Tyrosine Kinase 3 antibody Anti-P94-Fer antibody Anti-FER antibody Anti-TYK3 antibody |
Information sourced from Uniprot.org