Anti-Phospho-EEF2K-Ser366 antibody (331-380 aa) (STJ90759)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human eEF2K around the phosphorylation site of Ser366 at the amino acid range 331-380
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Eukaryotic elongation factor 2 kinase-Ser366 (331-380 aa) for use in WB, IHC, IF and ELISA in Human and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:40000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | EEF2K |
| Gene ID | 29904 |
| Uniprot ID | EF2K_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human eEF2K around the phosphorylation site of Ser366 at the amino acid range 331-380 |
| Immunogen Region | 331-380 aa |
| Specificity | Phospho-eEF2K (S366) Polyclonal Antibody detects endogenous levels of eEF2K protein only when phosphorylated at S366. |
Additional Info
| Function | Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced. |
| Protein Name | Eukaryotic Elongation Factor 2 KinaseEef-2 KinaseEef-2kCalcium/Calmodulin-Dependent Eukaryotic Elongation Factor 2 Kinase |
| Database Links | Reactome: R-HSA-166208 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-Eukaryotic Elongation Factor 2 Kinase antibodyAnti-Eef-2 Kinase antibodyAnti-Eef-2k antibodyAnti-Calcium/Calmodulin-Dependent Eukaryotic Elongation Factor 2 Kinase antibodyAnti-EEF2K antibody |
Information sourced from Uniprot.org