Anti-Phospho-EEF2K-Ser-398 antibody (STJA0005392)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenPhospho-eEF2K (Ser-398) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding Ser-398 in human eEF2K. This sequence is well conserved in rat and mouse eEF2K, and has low homology to other proteins.
General Information
| Short Description | Rabbit polyclonal antibody anti-Phospho-eEF2K-Ser-398 is suitable for use in Western Blot research applications. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Antigen Affinity Purified |
| Dilution Range | WB 1:1000 |
| Formulation | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | EEF2K |
| Gene ID | 29904 |
| Uniprot ID | EF2K_HUMAN |
| Immunogen | Phospho-eEF2K (Ser-398) synthetic peptide (coupled to carrier protein) corresponds to amino acids surrounding Ser-398 in human eEF2K. This sequence is well conserved in rat and mouse eEF2K, and has low homology to other proteins. |
| Specificity | The antibody was cross adsorbed to unphosphorylated eEF2K (Ser-398) peptide before affinity purification using phospho-eEF2K (Ser-398) peptide (without carrier). This antibody detects a 105 kDa* protein corresponding to the molecular mass of eEF2K on |
Additional Info
| Post Translational Modifications | Autophosphorylated at multiple residues, Thr-348 being the major site. Phosphorylated by AMP-activated protein kinase AMPK at Ser-398 leading to EEF2K activation and protein synthesis inhibition. Phosphorylated by TRPM7 at Ser-78 resulting in improved protein stability, higher EE2F phosphorylated and subsequently reduced rate of protein synthesis. Phosphorylation by other kinases such as CDK1 and MAPK13 at Ser-359 or RPS6KA1 and RPS6KB1 at Ser-366 instead decrease EEF2K activity and promote protein synthesis. |
| Function | Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced. |
| Protein Name | Eukaryotic Elongation Factor 2 KinaseEef-2 KinaseEef-2kCalcium/Calmodulin-Dependent Eukaryotic Elongation Factor 2 Kinase |
| Database Links | Reactome: R-HSA-166208 |
| Alternative Antibody Names | Anti-Eukaryotic Elongation Factor 2 Kinase antibodyAnti-Eef-2 Kinase antibodyAnti-Eef-2k antibodyAnti-Calcium/Calmodulin-Dependent Eukaryotic Elongation Factor 2 Kinase antibodyAnti-EEF2K antibody |
Information sourced from Uniprot.org