• Western blot analysis of extracts of various cell lines, using Phospho-DDR1-Y792 antibody (STJ11101110) at 1:1000 dilution. 293T cells were treated by ATP (5 mM) at 30 °C for 1 hour. NIH/3T3 cells were treated by ATP (5 mM) at 30 °C for 1 hour. C6 cells were treated by ATP (5 mM) at 30 °C for 1 hour. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of various lysates using Phospho-DDR1-Y792 Rabbit polyclonal antibody (STJ11101110) at 1:1000 dilution. 293T cells were treated by ATP (5 mM) at 30 °C for 1 hour. NIH/3T3 cells were treated by ATP (5 mM) at 30 °C for 1 hour. C6 cells were treated by ATP (5 mM) at 30 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Phospho-DDR1-Y792 antibody (STJ11101110)

SKU:
STJ11101110

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-DDR1-Y792 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DDR1
Gene ID: 780
Uniprot ID: DDR1_HUMAN
Immunogen: A synthetic phosphorylated peptide around Y792 of human DDR1 (NP_054699.2).
Immunogen Sequence: NLYAG
Tissue Specificity Detected in T-47D, MDA-MB-175 and HBL-100 breast carcinoma cells, A-431 epidermoid carcinoma cells, SW48 and SNU-C2B colon carcinoma cells and Hs 294T melanoma cells (at protein level). Expressed at low levels in most adult tissues and is highest in the brain, lung, placenta and kidney. Lower levels of expression are detected in melanocytes, heart, liver, skeletal muscle and pancreas. Abundant in breast carcinoma cell lines. In the colonic mucosa, expressed in epithelia but not in the connective tissue of the lamina propria. In the thyroid gland, expressed in the epithelium of the thyroid follicles. In pancreas, expressed in the islets of Langerhans cells, but not in the surrounding epithelial cells of the exocrine pancreas. In kidney, expressed in the epithelia of the distal tubules. Not expressed in connective tissue, endothelial cells, adipose tissue, muscle cells or cells of hematopoietic origin.
Post Translational Modifications Autophosphorylated in response to fibrillar collagen binding. Glycosylation of Asn-211, but apparently not of Asn-260 or Asn-394, prevents autophosphorylation from occurring in the absence of collagen.
Function Tyrosine kinase that functions as cell surface receptor for fibrillar collagen and regulates cell attachment to the extracellular matrix, remodeling of the extracellular matrix, cell migration, differentiation, survival and cell proliferation. Collagen binding triggers a signaling pathway that involves SRC and leads to the activation of MAP kinases. Regulates remodeling of the extracellular matrix by up-regulation of the matrix metalloproteinases MMP2, MMP7 and MMP9, and thereby facilitates cell migration and wound healing. Required for normal blastocyst implantation during pregnancy, for normal mammary gland differentiation and normal lactation. Required for normal ear morphology and normal hearing. Promotes smooth muscle cell migration, and thereby contributes to arterial wound healing. Also plays a role in tumor cell invasion. Phosphorylates PTPN11.
Protein Name Epithelial Discoidin Domain-Containing Receptor 1
Epithelial Discoidin Domain Receptor 1
Cd167 Antigen-Like Family Member A
Cell Adhesion Kinase
Discoidin Receptor Tyrosine Kinase
Hgk2
Mammary Carcinoma Kinase 10
Mck-10
Protein-Tyrosine Kinase 3a
Protein-Tyrosine Kinase Rtk-6
Trk E
Tyrosine Kinase Ddr
Tyrosine-Protein Kinase Cak
Cd Antigen Cd167a
Database Links Reactome: R-HSA-3000171
Cellular Localisation Isoform 1: Cell Membrane
Single-Pass Type I Membrane Protein
Isoform 2: Cell Membrane
Isoform 3: Secreted
Isoform 4: Cell Membrane
Alternative Antibody Names Anti-Epithelial Discoidin Domain-Containing Receptor 1 antibody
Anti-Epithelial Discoidin Domain Receptor 1 antibody
Anti-Cd167 Antigen-Like Family Member A antibody
Anti-Cell Adhesion Kinase antibody
Anti-Discoidin Receptor Tyrosine Kinase antibody
Anti-Hgk2 antibody
Anti-Mammary Carcinoma Kinase 10 antibody
Anti-Mck-10 antibody
Anti-Protein-Tyrosine Kinase 3a antibody
Anti-Protein-Tyrosine Kinase Rtk-6 antibody
Anti-Trk E antibody
Anti-Tyrosine Kinase Ddr antibody
Anti-Tyrosine-Protein Kinase Cak antibody
Anti-Cd Antigen Cd167a antibody
Anti-DDR1 antibody
Anti-CAK antibody
Anti-EDDR1 antibody
Anti-NEP antibody
Anti-NTRK4 antibody
Anti-PTK3A antibody
Anti-RTK6 antibody
Anti-TRKE antibody

Information sourced from Uniprot.org

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