Anti-Phospho-DAPK2-Ser318 antibody (284-333 aa) (STJ91302)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human DAPK2 around the phosphorylation site of Ser318 at the amino acid range 284-333
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Death-associated protein kinase 2-Ser318 (284-333 aa) for use in IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | IHC 1:100-1:300IF 1:200-1:1000ELISA 1:5000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DAPK2 |
| Gene ID | 23604 |
| Uniprot ID | DAPK2_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human DAPK2 around the phosphorylation site of Ser318 at the amino acid range 284-333 |
| Immunogen Region | 284-333 aa |
| Specificity | Phospho-DAPK2 (S318) Polyclonal Antibody detects endogenous levels of DAPK2 protein only when phosphorylated at S318. |
Additional Info
| Post Translational Modifications | Autophosphorylation at Ser-318 inhibits its catalytic activity. Dephosphorylated at Ser-318 in response to activated Fas and TNF-alpha receptors. |
| Function | Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell death signals, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Acts as a mediator of anoikis and a suppressor of beta-catenin-dependent anchorage-independent growth of malignant epithelial cells. May play a role in granulocytic maturation. Regulates granulocytic motility by controlling cell spreading and polarization. Isoform 2 is not regulated by calmodulin. It can phosphorylate MYL9. It can induce membrane blebbing and autophagic cell death. |
| Protein Name | Death-Associated Protein Kinase 2Dap Kinase 2Dap-Kinase-Related Protein 1Drp-1 |
| Database Links | Reactome: R-HSA-418889 |
| Cellular Localisation | CytoplasmCytoplasmic VesicleAutophagosome Lumen |
| Alternative Antibody Names | Anti-Death-Associated Protein Kinase 2 antibodyAnti-Dap Kinase 2 antibodyAnti-Dap-Kinase-Related Protein 1 antibodyAnti-Drp-1 antibodyAnti-DAPK2 antibody |
Information sourced from Uniprot.org