Anti-Phospho-DAB1-Tyr220 antibody (STJA0009871)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human Dab1 (Phospho Tyr220)
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Disabled homolog 1-Tyr220 for use in WB and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Dilution Range | WB 1:1000-2000ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | DAB1 |
| Gene ID | 1600 |
| Uniprot ID | DAB1_HUMAN |
| Immunogen | Synthesized peptide derived from human Dab1 (Phospho Tyr220) |
| Specificity | This antibody detects endogenous levels of Human, Mouse, Rat Dab1 (Phospho Tyr220) |
Additional Info
| Tissue Specificity | Mainly expressed in brain. |
| Post Translational Modifications | Phosphorylated by FYN on Tyr-198 and Tyr-220 upon reelin induction in embryonic neurons. Also phosphorylated on Ser-524 independently of reelin signaling. Ubiquitinated by various cullin-5-RING E3 ubiquitin-protein ligase complexes (ECS complexes) following ligand-binding and phosphorylation, leading to its degradation. Ubiquitinated by the ECS(SOCS7) complex in the cortical plate of the developing cerebral cortex following ligand-binding and phosphorylation by FYN, leading to its degradation by the proteasome. Recognized by ZSWIM8 through a disorder targets misorder mechanism that eliminates misfolded DAB1 via ubiquitination and proteasomal degradation. |
| Function | Signaling adapter of the reelin-mediated signaling pathway, which regulates the migration and differentiation of postmitotic neurons during brain development. Mediates intracellular transduction of Reelin signaling following reelin (RELN)-binding to its receptor: acts by docking proteins through its phosphotyrosine residues and PID domain. |
| Protein Name | Disabled Homolog 1 |
| Database Links | Reactome: R-HSA-8866376 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Disabled Homolog 1 antibodyAnti-DAB1 antibody |
Information sourced from Uniprot.org