Anti-Phospho-CREM antibody (STJ13100807)
SPECIFICATIONS
ClonalityPolyclonal
HostSheep
ConjugationUnconjugated
ImmunogenA synthetic peptide to phospho S271 & S274 of human CREM (cAMP-responsive element modulator, Inducible cAMP early repressor, ICER) conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat, mouse and dog.
General Information
| Short Description | Sheep polyclonal anti-Phospho-CREM for use in IHC and WB in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Sheep |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Conjugation | Unconjugated |
| Purification | Affinity purification |
| Dilution Range | IHC, WB. A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not tested in other applications. This antibody may also recognise the unphosphorylated form. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | CREM |
| Gene ID | 1390 |
| Uniprot ID | CREM_HUMAN |
| Immunogen | A synthetic peptide to phospho S271 & S274 of human CREM (cAMP-responsive element modulator, Inducible cAMP early repressor, ICER) conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat, mouse and dog. |
| Specificity | Specific for CREM. |
Additional Info
| Post Translational Modifications | Isoform 9 is ubiquitinated by CDC34 and RAD6B in order to be degraded by the proteasome. Stimulated by phosphorylation. Phosphorylated on Ser-116 by TSSK4 in vitro. |
| Function | Transcriptional regulator that binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. Isoforms are either transcriptional activators or repressors. Plays a role in spermatogenesis and is involved in spermatid maturation. Isoform 6: May play a role in the regulation of the circadian clock: acts as a transcriptional repressor of the core circadian component PER1 by directly binding to cAMP response elements in its promoter. |
| Protein Name | Camp-Responsive Element ModulatorInducible Camp Early RepressorIcer |
| Database Links | Reactome: R-HSA-400253 Q03060-6 |
| Cellular Localisation | NucleusIsoform 6: Cytoplasm |
| Alternative Antibody Names | Anti-Camp-Responsive Element Modulator antibodyAnti-Inducible Camp Early Repressor antibodyAnti-Icer antibodyAnti-CREM antibody |
Information sourced from Uniprot.org