| Host: | Rabbit |
| Applications: | WB/IF/ELISA |
| Reactivity: | Human/Mouse/Rat/Monkey |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-Phospho-Serine/threonine-protein kinase Chk2-Thr383 (356-405 aa) is suitable for use in Western Blot, Immunofluorescence and ELISA research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 IF 1:200-1:1000 ELISA 1:20000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CHEK2 |
| Gene ID: | 11200 |
| Uniprot ID: | CHK2_HUMAN |
| Immunogen Region: | 356-405 aa |
| Specificity: | Phospho-Chk2 (T383) Polyclonal Antibody detects endogenous levels of Chk2 protein only when phosphorylated at T383. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human Chk2 around the phosphorylation site of Thr383 at the amino acid range 356-405 |
| Post Translational Modifications | Phosphorylated. Phosphorylated at Ser-73 by PLK3 in response to DNA damage, promoting phosphorylation at Thr-68 by ATM and the G2/M transition checkpoint. Phosphorylation at Thr-68 induces homodimerization. Autophosphorylates at Thr-383 and Thr-387 in the T-loop/activation segment upon dimerization to become fully active and phosphorylate its substrates like for instance CDC25C. DNA damage-induced autophosphorylation at Ser-379 induces CUL1-mediated ubiquitination and regulates the pro-apoptotic function. Phosphorylation at Ser-456 also regulates ubiquitination. Phosphorylated by PLK4. Ubiquitinated. CUL1-mediated ubiquitination regulates the pro-apoptotic function. Ubiquitination may also regulate protein stability. Ubiquitinated by RNF8 via 'Lys-48'-linked ubiquitination. |
| Function | Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence L-X-R-X-X-S/T. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1. Regulates apoptosis through the phosphorylation of p53/TP53, MDM4 and PML. Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, leading to accumulation of active p53/TP53. Phosphorylation of MDM4 may also reduce degradation of p53/TP53. Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1. Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1. Its absence may be a cause of the chromosomal instability observed in some cancer cells. Promotes the CCAR2-SIRT1 association and is required for CCAR2-mediated SIRT1 inhibition. Under oxidative stress, promotes ATG7 ubiquitination by phosphorylating the E3 ubiquitin ligase TRIM32 at 'Ser-55' leading to positive regulation of the autophagosme assembly. (Microbial infection) Phosphorylates herpes simplex virus 1/HHV-1 protein ICP0 and thus activates its SUMO-targeted ubiquitin ligase activity. |
| Protein Name | Serine/Threonine-Protein Kinase Chk2 Chk2 Checkpoint Homolog Cds1 Homolog Hucds1 Hcds1 Checkpoint Kinase 2 |
| Database Links | Reactome: R-HSA-5693565 Reactome: R-HSA-6804756 Reactome: R-HSA-6804757 Reactome: R-HSA-6804760 Reactome: R-HSA-69473 Reactome: R-HSA-69541 Reactome: R-HSA-69601 Reactome: R-HSA-75035 |
| Cellular Localisation | Isoform 2: Nucleus Isoform 10 Is Present Throughout The Cell Isoform 4: Nucleus Isoform 7: Nucleus Isoform 9: Nucleus Isoform 12: Nucleus Nucleus Pml Body Nucleoplasm Recruited Into Pml Bodies Together With Tp53 |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase Chk2 antibody Anti-Chk2 Checkpoint Homolog antibody Anti-Cds1 Homolog antibody Anti-Hucds1 antibody Anti-Hcds1 antibody Anti-Checkpoint Kinase 2 antibody Anti-CHEK2 antibody Anti-CDS1 antibody Anti-CHK2 antibody Anti-RAD53 antibody |
Information sourced from Uniprot.org
