• Western blot analysis of lysates from HUVEC cells treated with serum 20% 30', using CDC25C (Phospho-Ser216) Antibody. The lane on the right is blocked with the phospho peptide.
  • Western blot analysis of HuvEc cells using Phospho-Cdc25C (S216) Polyclonal Antibody diluted at 1：1000
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CDC25C (Phospho-Ser216) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of various cells using Phospho-Cdc25C (S216) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-CDC25C-Ser216 antibody (183-232 aa) (STJ90218)

SKU:
STJ90218

$62.10 - $305.10
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-M-phase inducer phosphatase 3-Ser216 (183-232 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CDC25C
Gene ID: 995
Uniprot ID: MPIP3_HUMAN
Immunogen Region: 183-232 aa
Specificity: Phospho-Cdc25C (S216) Polyclonal Antibody detects endogenous levels of Cdc25C protein only when phosphorylated at S216.
Immunogen: The antiserum was produced against synthesized peptide derived from the human CDC25C around the phosphorylation site of Ser216 at the amino acid range 183-232
Function Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity.
Protein Name M-Phase Inducer Phosphatase 3
Dual Specificity Phosphatase Cdc25c
Database Links Reactome: R-HSA-156711
Reactome: R-HSA-176187
Reactome: R-HSA-5625740
Reactome: R-HSA-6804114
Reactome: R-HSA-6804115
Reactome: R-HSA-69273
Reactome: R-HSA-75035
Reactome: R-HSA-8862803
Cellular Localisation Nucleus
Alternative Antibody Names Anti-M-Phase Inducer Phosphatase 3 antibody
Anti-Dual Specificity Phosphatase Cdc25c antibody
Anti-CDC25C antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance