• Western blot analysis of lysates from NIH/3T3 cells treated with UV 15', using Cyclin C (Phospho-Ser275) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Cyclin C (Phospho-Ser275) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-CCNC-Ser275 antibody (234-283 aa) (STJ90976)

SKU:
STJ90976

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Cyclin-C-Ser275 (234-283 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CCNC
Gene ID: 892
Uniprot ID: CCNC_HUMAN
Immunogen Region: 234-283 aa
Specificity: Phospho-Cyclin C (S275) Polyclonal Antibody detects endogenous levels of Cyclin C protein only when phosphorylated at S275.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Cyclin C around the phosphorylation site of Ser275 at the amino acid range 234-283
Function Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Binds to and activates cyclin-dependent kinase CDK8 that phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex.
Protein Name Cyclin-C
Srb11 Homolog
Hsrb11
Database Links Reactome: R-HSA-1989781
Reactome: R-HSA-2122947
Reactome: R-HSA-212436
Reactome: R-HSA-2173796
Reactome: R-HSA-2644606
Reactome: R-HSA-2894862
Reactome: R-HSA-381340
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Cyclin-C antibody
Anti-Srb11 Homolog antibody
Anti-Hsrb11 antibody
Anti-CCNC antibody

Information sourced from Uniprot.org

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