Anti-Phospho-CCDC88A-Ser-1416 antibody (STJA0005428)

SKU:
STJA0005428-100

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Girdin-Ser-1416 is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Purification: Antigen Affinity Purified
Dilution Range: WB 1:500
IHC
ICC
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CCDC88A
Gene ID: 55704
Uniprot ID: GRDN_HUMAN
Specificity: The antibody was cross-adsorbed to unphosphorylated Girdin (Ser-1416) peptide before affinity purification using phospho-Girdin (Ser-1416) peptide. This antibody detects a 250 kDa* protein on SDS-PAGE immunoblots of human A431 cells stimulated with E
Immunogen: Phospho-Girdin (Ser-1416) synthetic peptide (coupled to carrier) corresponding to amino acid residues around serine 1416 in human Girdin. This sequence is identical in rat and mouse Girdin, and has low homology to similar regions in other Girdin fami
Tissue Specificity Expressed ubiquitously.
Post Translational Modifications Phosphorylation is induced by epidermal growth factor (EGF) in a phosphoinositide 3-kinase (PI3K)-dependent manner. Phosphorylation by AKT1/PKB is necessary for delocalization from the cell membrane and for cell migration. Phosphorylated on tyrosine residues which promotes binding to phosphatidylinositol 3-kinase (PI3K) regulatory subunit PIK3R1/p85a and enhances PI3K activity. Tyrosine-phosphorylated by both receptor and non-receptor tyrosine kinases in vitro. Tyrosine phosphorylation is required for AKT1-dependent phosphorylation of Ser-1417. Phosphorylation at Ser-1690 by PRKCQ disrupts interaction with GNAI3 and inhibits guanine nucleotide exchange factor activity.
Function Bifunctional modulator of guanine nucleotide-binding proteins (G proteins). Acts as a non-receptor guanine nucleotide exchange factor which binds to and activates guanine nucleotide-binding protein G(i) alpha subunits. Also acts as a guanine nucleotide dissociation inhibitor for guanine nucleotide-binding protein G(s) subunit alpha GNAS. Essential for cell migration. Interacts in complex with G(i) alpha subunits with the EGFR receptor, retaining EGFR at the cell membrane following ligand stimulation and promoting EGFR signaling which triggers cell migration. Binding to Gi-alpha subunits displaces the beta and gamma subunits from the heterotrimeric G-protein complex which enhances phosphoinositide 3-kinase (PI3K)-dependent phosphorylation and kinase activity of AKT1/PKB. Phosphorylation of AKT1/PKB induces the phosphorylation of downstream effectors GSK3 and FOXO1/FKHR, and regulates DNA replication and cell proliferation. Binds in its tyrosine-phosphorylated form to the phosphatidylinositol 3-kinase (PI3K) regulatory subunit PIK3R1 which enables recruitment of PIK3R1 to the EGFR receptor, enhancing PI3K activity and cell migration. Plays a role as a key modulator of the AKT-mTOR signaling pathway, controlling the tempo of the process of newborn neuron integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Inhibition of G(s) subunit alpha GNAS leads to reduced cellular levels of cAMP and suppression of cell proliferation. Essential for the integrity of the actin cytoskeleton. Required for formation of actin stress fibers and lamellipodia. May be involved in membrane sorting in the early endosome. Plays a role in ciliogenesis and cilium morphology and positioning and this may partly be through regulation of the localization of scaffolding protein CROCC/Rootletin.
Protein Name Girdin
Akt Phosphorylation Enhancer
Ape
Coiled-Coil Domain-Containing Protein 88a
G Alpha-Interacting Vesicle-Associated Protein
Giv
Girders Of Actin Filament
Hook-Related Protein 1
Hkrp1
Database Links Reactome: R-HSA-9696264
Reactome: R-HSA-9696273
Cellular Localisation Cell Membrane
Peripheral Membrane Protein
Cytoplasm
Cytosol
Cytoplasmic Vesicle
Cell Projection
Lamellipodium
Cytoskeleton
Cilium Basal Body
Microtubule Organizing Center
Centrosome
Centriole
Localizes To The Cytosol In Unstimulated Cells While Egf Stimulation Promotes Membrane Localization And Guanine Nucleotide Exchange Factor Activity
Localizes To The Cell Membrane Through Interaction With Phosphoinositides
Alternative Antibody Names Anti-Girdin antibody
Anti-Akt Phosphorylation Enhancer antibody
Anti-Ape antibody
Anti-Coiled-Coil Domain-Containing Protein 88a antibody
Anti-G Alpha-Interacting Vesicle-Associated Protein antibody
Anti-Giv antibody
Anti-Girders Of Actin Filament antibody
Anti-Hook-Related Protein 1 antibody
Anti-Hkrp1 antibody
Anti-CCDC88A antibody
Anti-APE antibody
Anti-GRDN antibody
Anti-KIAA1212 antibody

Information sourced from Uniprot.org

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