| Host: | Mouse |
| Applications: | WB/ICC |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Mouse monoclonal antibody anti-Phospho-Caveolin-1-Tyr-14 is suitable for use in Western Blot and Immunocytochemistry research applications. |
| Clonality: | Monoclonal |
| Clone ID: | M283 |
| Conjugation: | Unconjugated |
| Isotype: | IgG1 |
| Formulation: | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Purification: | Protein A Purified |
| Dilution Range: | WB 1:1000IHCICC 1:100IP |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | CAV1 |
| Gene ID: | 857 |
| Uniprot ID: | CAV1_HUMAN |
| Specificity: | This antibody detects a 21 kDa* protein corresponding to the apparent molecular mass of caveolin-1 on SDS-PAGE immunoblots of human A431 cells treated with EGF or pervanadate, and is not observed in untreated cells. |
| Immunogen: | Clone M283 was generated from a phospho-Caveolin-1 (Tyr-14) synthetic peptide corresponding to amino acids surrounding tyrosine 14 in human Caveolin-1. This sequence has significant homology to the conserved site in rat and mouse Caveolin-1. |
| Tissue Specificity | Skeletal muscle, liver, stomach, lung, kidney and heart (at protein level). Expressed in the brain. |
| Post Translational Modifications | Ubiquitinated. Undergo monoubiquitination and multi- and/or polyubiquitination. Monoubiquitination of N-terminal lysines promotes integration in a ternary complex with UBXN6 and VCP which promotes oligomeric CAV1 targeting to lysosomes for degradation. Ubiquitinated by ZNRF1.leading to degradation and modulation of the TLR4-mediated immune response. The initiator methionine for isoform 2 is removed during or just after translation. The new N-terminal amino acid is then N-acetylated. Phosphorylated at Tyr-14 by ABL1 in response to oxidative stress. |
| Function | May act as a scaffolding protein within caveolar membranes. Forms a stable heterooligomeric complex with CAV2 that targets to lipid rafts and drives caveolae formation. Mediates the recruitment of CAVIN proteins (CAVIN1/2/3/4) to the caveolae. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway. Negatively regulates TGFB1-mediated activation of SMAD2/3 by mediating the internalization of TGFBR1 from membrane rafts leading to its subsequent degradation. |
| Protein Name | Caveolin-1 |
| Database Links | Reactome: R-HSA-163560Reactome: R-HSA-203615Reactome: R-HSA-203641Reactome: R-HSA-210991Reactome: R-HSA-4641262Reactome: R-HSA-5218920Reactome: R-HSA-8980692Reactome: R-HSA-9009391Reactome: R-HSA-9013026Reactome: R-HSA-9013106Reactome: R-HSA-9013148Reactome: R-HSA-9013149Reactome: R-HSA-9013404Reactome: R-HSA-9013405Reactome: R-HSA-9013406Reactome: R-HSA-9013407Reactome: R-HSA-9013408Reactome: R-HSA-9013409Reactome: R-HSA-9013423Reactome: R-HSA-9035034Reactome: R-HSA-9617828Reactome: R-HSA-9696264Reactome: R-HSA-9696270Reactome: R-HSA-9696273Reactome: R-HSA-9735871Reactome: R-HSA-9755779 |
| Cellular Localisation | Golgi Apparatus MembranePeripheral Membrane ProteinCell MembraneMembraneCaveolaMembrane RaftGolgi ApparatusTrans-Golgi NetworkColocalized With Dpp4 In Membrane RaftsPotential Hairpin-Like Structure In The MembraneMembrane Protein Of Caveolae |
| Alternative Antibody Names | Anti-Caveolin-1 antibodyAnti-CAV1 antibodyAnti-CAV antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
