• Western blot of rat brain lysate showing specific immunolabeling of the ~50 kDa Alpha- and the ~60 kDa Beta-CaM Kinase II phosphorylated at Thr286 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase ( Lambda-Ptase, 1200 units for 30 minutes).

Anti-Phospho-CAMK2A/B-Thr286 antibody (STJA0003589)

SKU:
STJA0003589-100

£442.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rabbit/Rat/Zebrafish
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-CaM Kinase II-Thr286 is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 100 µl in 10 mM HEPES (pH 7.5) , 150 mM NaCl, 100 µg per ml BSA and 50% Glycerol.
Purification: This antibody was antigen affinity purified from pooled serum.
Dilution Range: WB 1:1000
IHC 1:1000
ICC
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Camk2a
Gene ID: 25400
Uniprot ID: KCC2A_RAT
Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr286 conjugated to KLH.
Post Translational Modifications Autophosphorylation of Thr-286 following activation by Ca(2+)/calmodulin. Phosphorylation of Thr-286 locks the kinase into an activated state. Palmitoylated. Probably palmitoylated by ZDHHC3 and ZDHHC7.
Function Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in various processes, such as synaptic plasticity, neurotransmitter release and long-term potentiation. Member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR and therefore excitatory synaptic transmission. Regulates dendritic spine development. Also regulates the migration of developing neurons. Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity. Phosphorylates the transcription factor ETS1 in response to calcium signaling, thereby decreasing ETS1 affinity for DNA. In response to interferon-gamma (IFN-gamma) stimulation, catalyzes phosphorylation of STAT1, stimulating the JAK-STAT signaling pathway. In response to interferon-beta (IFN-beta) stimulation, stimulates the JAK-STAT signaling pathway. Acts as a negative regulator of 2-arachidonoylglycerol (2-AG)-mediated synaptic signaling via modulation of DAGLA activity.
Protein Name Calcium/Calmodulin-Dependent Protein Kinase Type Ii Subunit Alpha
Cam Kinase Ii Subunit Alpha
Camk-Ii Subunit Alpha
Database Links Reactome: R-RNO-3371571
Reactome: -RNO-399719
Reactome: -RNO-4086398
Reactome: -RNO-438066
Reactome: -RNO-5578775
Reactome: -RNO-5673000
Reactome: -RNO-5673001
Reactome: -RNO-877300
Reactome: -RNO-936837
Cellular Localisation Synapse
Postsynaptic Density
Cell Projection
Dendritic Spine
Dendrite
Postsynaptic Lipid Rafts
Alternative Antibody Names Anti-Calcium/Calmodulin-Dependent Protein Kinase Type Ii Subunit Alpha antibody
Anti-Cam Kinase Ii Subunit Alpha antibody
Anti-Camk-Ii Subunit Alpha antibody
Anti-Camk2a antibody

Information sourced from Uniprot.org

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