Anti-Phospho-BRIP1-Thr1133 antibody (STJ196249)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized phosho peptide around human BACH1 and BRIP1 (Thr1133)
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Fanconi anemia group J protein-Thr1133 for use in WB in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen. |
| Dilution Range | WB 1:1000-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | BRIP1 |
| Gene ID | 83990 |
| Uniprot ID | FANCJ_HUMAN |
| Immunogen | Synthesized phosho peptide around human BACH1 and BRIP1 (Thr1133) |
| Specificity | This antibody detects endogenous levels of Human BACH1 or BRIP1 (phospho-Thr1133) |
Additional Info
| Post Translational Modifications | Phosphorylated. Phosphorylation is necessary for interaction with BRCA1, and is cell-cycle regulated. Acetylation at Lys-1249 facilitates DNA end processing required for repair and checkpoint signaling. |
| Function | DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of chromosomal stability. Acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination. Involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1. Involved in the repair of abasic sites at replication forks by promoting the degradation of DNA-protein cross-links: acts by catalyzing unfolding of HMCES DNA-protein cross-link via its helicase activity, exposing the underlying DNA and enabling cleavage of the DNA-protein adduct by the SPRTN metalloprotease. Can unwind RNA:DNA substrates. Unwinds G-quadruplex DNA.unwinding requires a 5'-single stranded tail. |
| Protein Name | Fanconi Anemia Group J ProteinBrca1-Associated C-Terminal Helicase 1Brca1-Interacting Protein C-Terminal Helicase 1Brca1-Interacting Protein 1Dna 5'-3' Helicase Fancj |
| Database Links | Reactome: R-HSA-2564830Reactome: R-HSA-5685938Reactome: R-HSA-5685942Reactome: R-HSA-5693554Reactome: R-HSA-5693568Reactome: R-HSA-5693579Reactome: R-HSA-5693607Reactome: R-HSA-5693616Reactome: R-HSA-6804756Reactome: R-HSA-69473Reactome: R-HSA-9701192Reactome: R-HSA-9704331Reactome: R-HSA-9704646Reactome: R-HSA-9709570Reactome: R-HSA-9709603 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-Fanconi Anemia Group J Protein antibodyAnti-Brca1-Associated C-Terminal Helicase 1 antibodyAnti-Brca1-Interacting Protein C-Terminal Helicase 1 antibodyAnti-Brca1-Interacting Protein 1 antibodyAnti-Dna 5'-3' Helicase Fancj antibodyAnti-BRIP1 antibodyAnti-BACH1 antibodyAnti-FANCJ antibody |
Information sourced from Uniprot.org