• Western blot analysis of extracts of HT-29 cells, using Phospho-BRCA1-S1423 antibody (STJ22043) at 1:2000 dilution. HT-29 cells were treated by Anisomycin (5ug/mL) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 5s.
  • Immunofluorescence analysis of U2OS cells using Phospho-BRCA1-S1423 antibody (STJ22043) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded rat testis using Phospho-BRCA1-S1423 antibody (STJ22043) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded mouse testis using Phospho-BRCA1-S1423 antibody (STJ22043) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human breast cancer using Phospho-BRCA1-S1423 antibody (STJ22043) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using Phospho-BRCA1-S1423 antibody (STJ22043) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse testis using Phospho-BRCA1-S1423 antibody (STJ22043) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded rat testis using Phospho-BRCA1-S1423 antibody (STJ22043) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of U2OS cells using Phospho-BRCA1-S1423 antibody (STJ22043) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of HT-29 cells, using Phospho-BRCA1-S1423 antibody (STJ22043) at 1:2000 dilution. HT-29 cells were treated by Anisomycin (5 Mu g/mL) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-Phospho-BRCA1-S1423 antibody (STJ22043)

SKU:
STJ22043

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-BRCA1-S1423 is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: BRCA1
Gene ID: 672
Uniprot ID: BRCA1_HUMAN
Immunogen: A synthetic phosphorylated peptide around S1423 of human BRCA1 (NP_009225.1).
Immunogen Sequence: HGSQP
Tissue Specificity Isoform 1 and isoform 3 are widely expressed. Isoform 3 is reduced or absent in several breast and ovarian cancer cell lines.
Post Translational Modifications Phosphorylated in response to IR, UV, and various stimuli that cause checkpoint activation, probably by ATM or ATR. Phosphorylation at Ser-988 by CHEK2 regulates mitotic spindle assembly. Phosphorylation by AURKA regulates centrosomal microtubule nucleation. Autoubiquitinated, undergoes 'Lys-6'-linked polyubiquitination. 'Lys-6'-linked polyubiquitination does not promote degradation.
Function E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. It is unclear whether it also mediates the formation of other types of polyubiquitin chains. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Regulates centrosomal microtubule nucleation. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Required for FANCD2 targeting to sites of DNA damage. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation. Contributes to homologous recombination repair (HRR) via its direct interaction with PALB2, fine-tunes recombinational repair partly through its modulatory role in the PALB2-dependent loading of BRCA2-RAD51 repair machinery at DNA breaks. Component of the BRCA1-RBBP8 complex which regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage via BRCA1-mediated ubiquitination of RBBP8. Acts as a transcriptional activator.
Protein Name Breast Cancer Type 1 Susceptibility Protein
Ring Finger Protein 53
Ring-Type E3 Ubiquitin Transferase Brca1
Database Links Reactome: R-HSA-1221632
Reactome: R-HSA-3108214
Reactome: R-HSA-5685938
Reactome: R-HSA-5685942
Reactome: R-HSA-5689901
Reactome: R-HSA-5693554
Reactome: R-HSA-5693565
Reactome: R-HSA-5693568
Reactome: R-HSA-5693571
Reactome: R-HSA-5693579
Reactome: R-HSA-5693607
Reactome: R-HSA-5693616
Reactome: R-HSA-6796648
Reactome: R-HSA-6804756
Reactome: R-HSA-69473
Reactome: R-HSA-8951664
Reactome: R-HSA-8953750
Reactome: R-HSA-912446
Reactome: R-HSA-9663199
Reactome: R-HSA-9699150
Reactome: R-HSA-9701192
Reactome: R-HSA-9704331
Reactome: R-HSA-9704646
Reactome: R-HSA-9709570
Reactome: R-HSA-9709603
Reactome: R-HSA-9755511
Cellular Localisation Nucleus
Chromosome
Cytoplasm
Localizes At Sites Of Dna Damage At Double-Strand Breaks (Dsbs)
Recruitment To Dna Damage Sites Is Mediated By Abraxas1 And The Brca1-A Complex
Translocated To The Cytoplasm During Uv-Induced Apoptosis
Isoform 3: Cytoplasm
Isoform 5: Cytoplasm
Alternative Antibody Names Anti-Breast Cancer Type 1 Susceptibility Protein antibody
Anti-Ring Finger Protein 53 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Brca1 antibody
Anti-BRCA1 antibody
Anti-RNF53 antibody

Information sourced from Uniprot.org

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