Anti-Phospho-Bad-Ser-112 antibody (STJA0041085)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenPhospho-Bad (Ser-112) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding serine 112 in mouse Bad. This peptide sequence is highly conserved in human (Ser-75) and rat (Ser-113) Bad.
General Information
| Short Description | Rabbit polyclonal anti-Phospho-Bad-Ser-112 for use in ELISA and WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ELISA/WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Antigen Affinity Purified |
| Dilution Range | WB 1:500 |
| Formulation | PBS + 0.05% NaN3 |
| Storage Instruction | Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at-20°C C or-80°C C, while keeping a working aliquot stored at 4°C C for short term. Avoid freeze/t |
Target Information
| Gene Symbol | Bad |
| Gene ID | 12015 |
| Uniprot ID | BAD_MOUSE |
| Immunogen | Phospho-Bad (Ser-112) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding serine 112 in mouse Bad. This peptide sequence is highly conserved in human (Ser-75) and rat (Ser-113) Bad. |
| Immunogen Sequence | Mouse |
| Specificity | The antibody detects a 23 kDa* doublet corresponding to the apparent molecular mass of phosphorylated Bad on SDS-PAGE immunoblots of mouse J774A.1 treated with calyculin A. This reactivity is not observed after lambda phosphatase treatment. |
Additional Info
| Post Translational Modifications | Phosphorylated on one or more of Ser-112, Ser-136, Ser-155 and Ser-170 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-136 or Ser-112 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-155, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-136 is the major site of AKT/PKB phosphorylation, Ser-155 the major site of protein kinase A (CAPK) phosphorylation. Methylation at Arg-131 and Arg-133 by PRMT1 inhibits Akt-mediated phosphorylation at Ser-136. |
| Function | Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. Appears to act as a link between growth factor receptor signaling and the apoptotic pathways. |
| Protein Name | Bcl2-Associated Agonist Of Cell DeathBadBcl-2-Binding Component 6Bcl-Xl/Bcl-2-Associated Death PromoterBcl2 Antagonist Of Cell Death |
| Database Links | Reactome: R-MMU-111447Reactome: -MMU-111453Reactome: -MMU-193648 |
| Cellular Localisation | Mitochondrion Outer MembraneCytoplasmColocalizes With Hif3a Isoform 2 In The CytoplasmUpon PhosphorylationLocates To The Cytoplasm |
| Alternative Antibody Names | Anti-Bcl2-Associated Agonist Of Cell Death antibodyAnti-Bad antibodyAnti-Bcl-2-Binding Component 6 antibodyAnti-Bcl-Xl/Bcl-2-Associated Death Promoter antibodyAnti-Bcl2 Antagonist Of Cell Death antibodyAnti-Bad antibodyAnti-Bbc6 antibody |
Information sourced from Uniprot.org