• Western blot analysis of extracts of various cell lines, using Phospho-AKT1-S473+AKT2-S474+AKT3-S472 antibody (STJ11101106) at 1:1000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. C6 cells were treated by 10% FBS at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of Phospho-Akt-S473 in paraffin-embedded mouse pancreas using Phospho-AKT1-S473+AKT2-S474+AKT3-S472 Rabbit polyclonal antibody (STJ11101106) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat lung using Phospho-AKT1-S473+AKT2-S474+AKT3-S472 rabbit polyclonal antibody (STJ11101106) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of Phospho-Akt-S473 in paraffin-embedded human colon carcinoma using Phospho-AKT1-S473+AKT2-S474+AKT3-S472 Rabbit polyclonal antibody (STJ11101106) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human colon carcinoma using Phospho-AKT1-S473+AKT2-S474+AKT3-S472 rabbit polyclonal antibody (STJ11101106) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of Phospho-Akt-S473 in paraffin-embedded rat lung using Phospho-AKT1-S473+AKT2-S474+AKT3-S472 Rabbit polyclonal antibody (STJ11101106) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse pancreas using Phospho-AKT1-S473+AKT2-S474+AKT3-S472 rabbit polyclonal antibody (STJ11101106) at dilution of 1:100 (40x lens).
  • Western blot analysis of lysates from Jurkat cells using Phospho-Akt-S473 Rabbit polyclonal antibody (STJ11101106) at 1:500 dilution. Jurkat cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 10s.
  • Western blot analysis of lysates from NIH/3T3 cells using Phospho-Akt-S473 Rabbit polyclonal antibody (STJ11101106) at 1:500 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 45s.
  • Western blot analysis of lysates from C6 cells using Phospho-Akt-S473 Rabbit polyclonal antibody (STJ11101106) at 1:500 dilution. C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25  Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 10s.

Anti-Phospho-AKT1/AKT2/AKT3-S473/S474/S472 antibody (STJ11101106)

SKU:
STJ11101106

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-AKT1/AKT2/AKT3-S473/S474/S472 is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: A synthetic phosphorylated peptide around S473 of human AKT1/AKT2/AKT3AKT1 (NP_005154.2).
Immunogen Sequence: QFSYS
Background Predicted to be involved in cell division. Located in nucleolus and nucleoplasm.

Information sourced from Uniprot.org

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