• Western blot analysis of lysates from HeLa cells treated with Forskolin 40nM 30', using ADD1 (Phospho-Ser726) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ADD1 (Phospho-Ser726) Antibody. The picture on the right is blocked with the phospho peptide.
  • Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Immunofluorescence analysis of HeLa cells, using ADD1 (Phospho-Ser726) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of various cells using Phospho-Adducin Alpha/Beta (S726/713) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-Adducin Alpha/Beta-Ser726/713 antibody (688-737 aa) (STJ91014)

SKU:
STJ91014

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Alpha-adducin and Beta-adducin-Ser726/713 (688-737 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:40000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ADD2
ADD1
Gene ID: 119
118
Uniprot ID: ADDB_HUMAN
ADDA_HUMAN
Immunogen Region: 688-737 aa
Specificity: Phospho-Adducin Alpha/Beta (S726/713) Polyclonal Antibody detects endogenous levels of Adducin Alpha/Beta protein only when phosphorylated at S726/713.
Immunogen: The antiserum was produced against synthesized peptide derived from the human ADD1 around the phosphorylation site of Ser726 at the amino acid range 688-737
Function Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
Protein Name Alpha-Adducin
Erythrocyte Adducin Subunit Alpha
Database Links Reactome: R-HSA-264870
Reactome: R-HSA-381038
Reactome: R-HSA-5223345
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Alternative Antibody Names Anti-Alpha-Adducin antibody
Anti-Erythrocyte Adducin Subunit Alpha antibody
Anti-ADD1 antibody
Anti-ADDA antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance