• STJ71424 (1µg/ml) staining of Human Spleen (A) , Tonsil (B) and (0. 1ug/ml) PBM (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ71424 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. Actin filaments were stained with phalloidin (red). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ71424 Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ71424 Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-Phorbolin 1/APOBEC3A antibody (Internal) (STJ71424)

SKU:
STJ71424-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IF/FC/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Phorbolin 1/APOBEC3A (Internal) is suitable for use in ELISA, Western Blot, Immunofluorescence, Flow Cytometry and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IHC-3-6µg/ml
IF-Strong expression of the protein seen in the nuclei of A431 and A549 cells. 10µg/ml
ELISA-antibody detection limit dilution 1:8000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Immunogen Region: Internal
Accession Number: NP_663745.1
Immunogen Sequence: TSNFNNGIGRHKTY
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance