Anti-PHLPP2 antibody (STJA0038036)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from human PHLPP2
General Information
| Short Description | Rabbit monoclonal anti-PHLPP2 for use in IHC-P in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC-P |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Purified |
| Dilution Range | IHC 1:100-1:200 |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | PHLPP2 |
| Gene ID | 23035 |
| Uniprot ID | PHLP2_HUMAN |
| Immunogen | A synthesized peptide derived from human PHLPP2 |
Additional Info
| Tissue Specificity | In colorectal cancer tissue, expression is highest in the surface epithelium of normal colonic mucosa adjacent to the cancer tissue but is largely excluded from the crypt bases. Expression is lost or significantly decreased in 80% of tested tumors (at protein level). |
| Function | Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family.specifically acts on 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of Akt triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylates STK4 on 'Thr-387' leading to STK4 activation and apoptosis. Dephosphorylates RPS6KB1 and is involved in regulation of cap-dependent translation. Inhibits cancer cell proliferation and may act as a tumor suppressor. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Dephosphorylates RAF1 inhibiting its kinase activity. |
| Protein Name | Ph Domain Leucine-Rich Repeat-Containing Protein Phosphatase 2Ph Domain Leucine-Rich Repeat-Containing Protein Phosphatase-LikePhlpp-Like |
| Database Links | Reactome: R-HSA-199418 |
| Cellular Localisation | CytoplasmMembranePeripheral Membrane ProteinNucleusIn Colorectal Cancer TissueExpression Is Concentrated In The Cytoplasm And Nucleus |
| Alternative Antibody Names | Anti-Ph Domain Leucine-Rich Repeat-Containing Protein Phosphatase 2 antibodyAnti-Ph Domain Leucine-Rich Repeat-Containing Protein Phosphatase-Like antibodyAnti-Phlpp-Like antibodyAnti-PHLPP2 antibodyAnti-KIAA0931 antibodyAnti-PHLPPL antibody |
Information sourced from Uniprot.org