Anti-PHLPP2 antibody (C-Term) (STJ71236)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-PHLPP2 (C-Term) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 140kDa band observed in Human Brain (Amygdala, Hippocampus, Substantia Nigra) lysates (calculated MW of 134kDa according to NP_055835.2). Recommended concentration: 0.1-0.3µg/ml |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | PHLPP2 |
| Gene ID | 23035 |
| Uniprot ID | PHLP2_HUMAN |
| Accession Number | NP_055835.2 |
| Immunogen Region | C-Term |
| Immunogen Sequence | PHEEDRTEPPEEFD |
Additional Info
| Function | Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family.specifically acts on 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of Akt triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylates STK4 on 'Thr-387' leading to STK4 activation and apoptosis. Dephosphorylates RPS6KB1 and is involved in regulation of cap-dependent translation. Inhibits cancer cell proliferation and may act as a tumor suppressor. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Dephosphorylates RAF1 inhibiting its kinase activity. |
| Protein Name | Ph Domain Leucine-Rich Repeat-Containing Protein Phosphatase 2Ph Domain Leucine-Rich Repeat-Containing Protein Phosphatase-LikePhlpp-Like |
| Database Links | Reactome: R-HSA-199418 |
| Cellular Localisation | CytoplasmMembranePeripheral Membrane ProteinNucleusIn Colorectal Cancer TissueExpression Is Concentrated In The Cytoplasm And Nucleus |
| Alternative Antibody Names | Anti-Ph Domain Leucine-Rich Repeat-Containing Protein Phosphatase 2 antibodyAnti-Ph Domain Leucine-Rich Repeat-Containing Protein Phosphatase-Like antibodyAnti-Phlpp-Like antibodyAnti-PHLPP2 antibodyAnti-KIAA0931 antibodyAnti-PHLPPL antibody |
Information sourced from Uniprot.org