Anti-PHLPP1 antibody (STJ193083)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from part of the human protein
General Information
| Short Description | Rabbit polyclonal anti-PH domain leucine-rich repeat-containing protein phosphatase 1 for use in WB and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PHLPP1 |
| Gene ID | 23239 |
| Uniprot ID | PHLP1_HUMAN |
| Immunogen | Synthesized peptide derived from part of the human protein |
| Specificity | PHLP1 Polyclonal Antibody detects endogenous levels of protein. |
Additional Info
| Function | Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family.specifically acts on 'Ser-473' of AKT2 and AKT3, 'Ser-660' of PRKCB and 'Ser-657' of PRKCA. Isoform 2 seems to have a major role in regulating Akt signaling in hippocampal neurons. Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of Akt triggers apoptosis and suppression of tumor growth. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Dephosphorylates STK4 on 'Thr-387' leading to STK4 activation and apoptosis. Dephosphorylates RPS6KB1 and is involved in regulation of cap-dependent translation. Inhibits cancer cell proliferation and may act as a tumor suppressor. Dephosphorylates RAF1 inhibiting its kinase activity. May act as a negative regulator of K-Ras signaling in membrane rafts. Involved in the hippocampus-dependent long-term memory formation. Involved in circadian control by regulating the consolidation of circadian periodicity after resetting. Involved in development and function of regulatory T-cells. |
| Protein Name | Ph Domain Leucine-Rich Repeat-Containing Protein Phosphatase 1Pleckstrin Homology Domain-Containing Family E Member 1Ph Domain-Containing Family E Member 1Suprachiasmatic Nucleus Circadian Oscillatory ProteinHscop |
| Database Links | Reactome: R-HSA-199418 |
| Cellular Localisation | CytoplasmMembranePeripheral Membrane ProteinNucleusIn Colorectal Cancer TissueExpression Is Concentrated At The Lateral Membrane Of Epithelial CellsIsoform 2: Cell Membrane |
| Alternative Antibody Names | Anti-Ph Domain Leucine-Rich Repeat-Containing Protein Phosphatase 1 antibodyAnti-Pleckstrin Homology Domain-Containing Family E Member 1 antibodyAnti-Ph Domain-Containing Family E Member 1 antibodyAnti-Suprachiasmatic Nucleus Circadian Oscillatory Protein antibodyAnti-Hscop antibodyAnti-PHLPP1 antibodyAnti-KIAA0606 antibodyAnti-PHLPP antibodyAnti-PLEKHE1 antibodyAnti-SCOP antibody |
Information sourced from Uniprot.org