• HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human PHLDA3 with C-terminal MYC tag probed with STJ70467 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with STJ70467 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

Anti-PHLDA3/TIH1 antibody (C-Term) (STJ70467)

SKU:
STJ70467-100

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Host: Goat
Applications: Pep-ELISA/WB-Trf
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-PHLDA3/TIH1 (C-Term) is suitable for use in ELISA and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: ELISA-antibody detection limit dilution 1:16000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: PHLDA3
Gene ID: 23612
Uniprot ID: PHLA3_HUMAN
Immunogen Region: C-Term
Accession Number: NP_036528.1
Immunogen Sequence: RARQSLGTGTLVS
Function p53/TP53-regulated repressor of Akt/AKT1 signaling. Represses AKT1 by preventing AKT1-binding to membrane lipids, thereby inhibiting AKT1 translocation to the cellular membrane and activation. Contributes to p53/TP53-dependent apoptosis by repressing AKT1 activity. Its direct transcription regulation by p53/TP53 may explain how p53/TP53 can negatively regulate AKT1. May act as a tumor suppressor.
Protein Name Pleckstrin Homology-Like Domain Family A Member 3
Tdag51/Ipl Homolog 1
Cellular Localisation Cytoplasm
Membrane
Peripheral Membrane Protein
Alternative Antibody Names Anti-Pleckstrin Homology-Like Domain Family A Member 3 antibody
Anti-Tdag51/Ipl Homolog 1 antibody
Anti-PHLDA3 antibody
Anti-TIH1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance