• Western blot analysis of various lysates using PHKA1 Rabbit polyclonal antibody (STJ118146) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PHKA1 antibody (600-820) (STJ118146)

SKU:
STJ118146

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PHKA1 (600-820) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PHKA1
Gene ID: 5255
Uniprot ID: KPB1_HUMAN
Immunogen Region: 600-820
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 600-820 of human PHKA1 (NP_001116142.1).
Immunogen Sequence: QTGKLSEFLTTSCCTHLSFM DPGPEGKLYSEDYDDNYDYL ESGNWMNDYDSTSHARCGDE VARYLDHLLAHTAPHPKLAP TSQKGGLDRFQAAVQTTCDL MSLVTKAKELHVQNVHMYLP TKLFQASRPSFNLLDSPHPR QENQVPSVRVEIHLPRDQSG EVDFKALVLQLKETSSLQEQ ADILYMLYTMKGPDWNTELY NERSATVRELLTELYGKVGE I
Tissue Specificity Muscle specific. Isoform 1 is predominant in vastus lateralis muscle. Isoform 2 predominates slightly in heart, and it predominates clearly in the other tissues tested.
Post Translational Modifications Although the final Cys may be farnesylated, the terminal tripeptide is probably not removed, and the C-terminus is not methylated.
Function Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The alpha chain may bind calmodulin.
Protein Name Phosphorylase B Kinase Regulatory Subunit Alpha - Skeletal Muscle Isoform
Phosphorylase Kinase Alpha M Subunit
Database Links Reactome: R-HSA-70221
Cellular Localisation Cell Membrane
Lipid-Anchor
Cytoplasmic Side
Alternative Antibody Names Anti-Phosphorylase B Kinase Regulatory Subunit Alpha - Skeletal Muscle Isoform antibody
Anti-Phosphorylase Kinase Alpha M Subunit antibody
Anti-PHKA1 antibody
Anti-PHKA antibody

Information sourced from Uniprot.org

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