Anti-PHKA1 antibody (600-820) (STJ118146)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PHKA1 (600-820) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PHKA1 |
| Gene ID | 5255 |
| Uniprot ID | KPB1_HUMAN |
| Immunogen Region | 600-820 |
| Immunogen Sequence | QTGKLSEFLTTSCCTHLSFM DPGPEGKLYSEDYDDNYDYL ESGNWMNDYDSTSHARCGDE VARYLDHLLAHTAPHPKLAP TSQKGGLDRFQAAVQTTCDL MSLVTKAKELHVQNVHMYLP TKLFQASRPSFNLLDSPHPR QENQVPSVRVEIHLPRDQSG EVDFKALVLQLKETSSLQEQ ADILYMLYTMKGPDWNTELY NERSATVRELLTELYGKVGE I |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 600-820 of human PHKA1 (NP_001116142.1). |
Additional Info
| Tissue Specificity | Muscle specific. Isoform 1 is predominant in vastus lateralis muscle. Isoform 2 predominates slightly in heart, and it predominates clearly in the other tissues tested. |
| Post Translational Modifications | Although the final Cys may be farnesylated, the terminal tripeptide is probably not removed, and the C-terminus is not methylated. |
| Function | Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The alpha chain may bind calmodulin. |
| Protein Name | Phosphorylase B Kinase Regulatory Subunit Alpha - Skeletal Muscle IsoformPhosphorylase Kinase Alpha M Subunit |
| Database Links | Reactome: R-HSA-70221 |
| Cellular Localisation | Cell MembraneLipid-AnchorCytoplasmic Side |
| Alternative Antibody Names | Anti-Phosphorylase B Kinase Regulatory Subunit Alpha - Skeletal Muscle Isoform antibodyAnti-Phosphorylase Kinase Alpha M Subunit antibodyAnti-PHKA1 antibodyAnti-PHKA antibody |
Information sourced from Uniprot.org