• Immunofluorescence analysis of MCF-7 cells using PHIP antibody (STJ29287).
  • Immunohistochemistry analysis of paraffin-embedded mouse tumor using PHIP antibody (STJ29287) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human kidney cancer using PHIP antibody (STJ29287) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human well-differentiated squamous skin carcinoma using PHIP antibody (STJ29287) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using PHIP antibody (STJ29287) at 1:1000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 90s.

Anti-PHIP antibody (1542-1821) (STJ29287)

SKU:
STJ29287

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PHIP (1542-1821) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PHIP
Gene ID: 55023
Uniprot ID: PHIP_HUMAN
Immunogen Region: 1542-1821
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1542-1821 of human PHIP (NP_060404.3).
Immunogen Sequence: KTILENSVKHSKALNTLSSP GQSSFSHGTRNNSAKENMEK EKPVKRKMKSSVLPKASTLS KSSAVIEQGDCKNNALVPGT IQVNGHGGQPSKLVKRGPGR KPKVEVNTNSGEIIHKKRGR KPKKLQYAKPEDLEQNNVHP IRDEVLPSSTCNFLSETNNV KEDLLQKKNRGGRKPKRKMK TQKLDADLLVPASVKVLRRS NRKKIDDPIDEEEEFEELKG SEPHMRTRNQGRRTAFYNE
Tissue Specificity Expressed in myeloma and epidermoid carcinoma cell lines.
Function Probable regulator of the insulin and insulin-like growth factor signaling pathways. Stimulates cell proliferation through regulation of cyclin transcription and has an anti-apoptotic activity through AKT1 phosphorylation and activation. Plays a role in the regulation of cell morphology and cytoskeletal organization.
Protein Name Ph-Interacting Protein
Phip
Ddb1- And Cul4-Associated Factor 14
Irs-1 Ph Domain-Binding Protein
Wd Repeat-Containing Protein 11
Database Links Reactome: R-HSA-9013418
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Ph-Interacting Protein antibody
Anti-Phip antibody
Anti-Ddb1- And Cul4-Associated Factor 14 antibody
Anti-Irs-1 Ph Domain-Binding Protein antibody
Anti-Wd Repeat-Containing Protein 11 antibody
Anti-PHIP antibody
Anti-DCAF14 antibody
Anti-WDR11 antibody

Information sourced from Uniprot.org

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