Anti-PHF2 antibody (Internal Region) (STJA0005669)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthetic peptide corresponding to residues from an internal region of the 120 kDa PHF protein
General Information
| Short Description | Rabbit polyclonal anti-PHF2 (Internal Region) for use in WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Antigen Affinity Purified |
| Dilution Range | WB 1:500-1:1000 |
| Formulation | PBS + 0.02% NaN3 |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PHF2 |
| Gene ID | 5253 |
| Uniprot ID | PHF2_HUMAN |
| Immunogen | Synthetic peptide corresponding to residues from an internal region of the 120 kDa PHF protein |
| Immunogen Region | Internal Region |
| Specificity | Reacts with residues from an internal region of the 120 kDa PHF protein. |
Additional Info
| Post Translational Modifications | Phosphorylated by PKA on specific serine residues, leading to the formation of an active lysine demethylase complex. |
| Function | Lysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA. Involved in the activation of toll-like receptor 4 (TLR4)-target inflammatory genes in macrophages by catalyzing the demethylation of trimethylated histone H4 lysine 20 (H4K20me3) at the gene promoters. |
| Protein Name | Lysine-Specific Demethylase Phf2Grc5Phd Finger Protein 2 |
| Database Links | Reactome: R-HSA-3214842 |
| Cellular Localisation | NucleusNucleolusChromosomeCentromereKinetochore |
| Alternative Antibody Names | Anti-Lysine-Specific Demethylase Phf2 antibodyAnti-Grc5 antibodyAnti-Phd Finger Protein 2 antibodyAnti-PHF2 antibodyAnti-CENP-35 antibodyAnti-KIAA0662 antibody |
Information sourced from Uniprot.org