| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PHF1 (1-260) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PHF1 |
| Gene ID: |
5252 |
| Uniprot ID: |
PHF1_HUMAN |
| Immunogen Region: |
1-260 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PHF1 (NP_077084.1). |
| Immunogen Sequence: |
MAQPPRLSRSGASSLWDPAS PAPTSGPRPRLWEGQDVLAR WTDGLLYLGTIKKVDSAREV CLVQFEDDSQFLVLWKDISP AALPGEELLCCVCRSETVVP GNRLVSCEKCRHAYHQDCHV PRAPAPGEGEGTSWVCRQCV FAIATKRGGALKKGPYARAM LGMKLSLPYGLKGLDWDAGH LSNRQQSYCYCGGPGEWNLK MLQCRSCLQWFHEACTQCLS KPLLYGDRFYEFECCVCRG |
| Tissue Specificity | Highest levels in heart, skeletal muscle, and pancreas, lower levels in brain, placenta, lung, liver and kidney. |
| Function | Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. Involved in DNA damage response and is recruited at double-strand breaks (DSBs). Acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex: it is however unclear whether recruitment of the PRC2 complex to H3K36me3 leads to enhance or inhibit H3K27me3 methylation mediated by the PRC2 complex. According to some reports, PRC2 recruitment by PHF1 promotes H3K27me3 and subsequent gene silencing by inducing spreading of PRC2 and H3K27me3 into H3K36me3 loci. According to another report, PHF1 recruits the PRC2 complex at double-strand breaks (DSBs) and inhibits the activity of PRC2. Regulates p53/TP53 stability and prolonges its turnover: may act by specifically binding to a methylated from of p53/TP53. |
| Protein Name | Phd Finger Protein 1Protein Phf1Hphf1Polycomb-Like Protein 1Hpcl1 |
| Database Links | Reactome: R-HSA-212300 |
| Cellular Localisation | NucleusCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeLocalizes Specifically To The Promoters Of Numerous Target GenesLocalizes To Double-Strand Breaks (Dsbs) Sites Following Dna DamageCo-Localizes With Nek6 In The Centrosome |
| Alternative Antibody Names | Anti-Phd Finger Protein 1 antibodyAnti-Protein Phf1 antibodyAnti-Hphf1 antibodyAnti-Polycomb-Like Protein 1 antibodyAnti-Hpcl1 antibodyAnti-PHF1 antibodyAnti-PCL1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
