• Western blot analysis of extracts of various cell lines, using PHC3 Antibody (STJ28562) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-PHC3 antibody (100-300) (STJ28562)

SKU:
STJ28562

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PHC3 (100-300) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PHC3
Gene ID: 80012
Uniprot ID: PHC3_HUMAN
Immunogen Region: 100-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 100-300 of human PHC3 (NP_079223.3).
Immunogen Sequence: LSSSQLQSLAAVQASLSSGR PSTSPTGSVTQQSSMSQTSI NLSTSPTPAQLISRSQASSS TSGSITQQTMLLGSTSPTLT ASQAQMYLRAQMLIFTPATT VAAVQSDIPVVSSSSSSSCQ SAATQVQNLTLRSQKLGVLS SSQNGPPKSTSQTQSLTICH NKTTVTSSKISQRDPSPESN KKGESPSLESRSTAVTRTSS I
Function Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones.it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
Protein Name Polyhomeotic-Like Protein 3
Early Development Regulatory Protein 3
Homolog Of Polyhomeotic 3
Hph3
Database Links Reactome: R-HSA-2559580
Reactome: R-HSA-3108214
Reactome: R-HSA-3899300
Reactome: R-HSA-4551638
Reactome: R-HSA-4570464
Reactome: R-HSA-4655427
Reactome: R-HSA-8939243
Reactome: R-HSA-8943724
Reactome: R-HSA-8953750
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Polyhomeotic-Like Protein 3 antibody
Anti-Early Development Regulatory Protein 3 antibody
Anti-Homolog Of Polyhomeotic 3 antibody
Anti-Hph3 antibody
Anti-PHC3 antibody
Anti-EDR3 antibody
Anti-PH3 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance