• Western blot analysis of extracts of various cell lines, using Prohibitin rabbit polyclonal antibody antibody (STJ24977) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using Prohibitin rabbit polyclonal antibody (STJ24977) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using Prohibitin rabbit polyclonal antibody (STJ24977) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using Prohibitin rabbit polyclonal antibody (STJ24977) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using Prohibitin rabbit polyclonal antibody (STJ24977) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of L929 cells using Prohibitin rabbit polyclonal antibody (STJ24977) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-PHB antibody (73-272) (STJ24977)

SKU:
STJ24977

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PHB (73-272) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PHB1
Gene ID: 5245
Uniprot ID: PHB1_HUMAN
Immunogen Region: 73-272
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 73-272 of human Prohibitin (NP_002625.1).
Immunogen Sequence: PRNVPVITGSKDLQNVNITL RILFRPVASQLPRIFTSIGE DYDERVLPSITTEILKSVVA RFDAGELITQRELVSRQVSD DLTERAATFGLILDDVSLTH LTFGKEFTEAVEAKQVAQQE AERARFVVEKAEQQKKAAII SAEGDSKAAELIANSLATAG DGLIELRKLEAAEDIAYQLS RSRNITYLPAGQSVLLQLPQ
Tissue Specificity Widely expressed in different tissues.
Function Protein with pleiotropic attributes mediated in a cell-compartment- and tissue-specific manner, which include the plasma membrane-associated cell signaling functions, mitochondrial chaperone, and transcriptional co-regulator of transcription factors in the nucleus. Plays a role in adipose tissue and glucose homeostasis in a sex-specific manner. Contributes to pulmonary vascular remodeling by accelerating proliferation of pulmonary arterial smooth muscle cells. In the mitochondria, together with PHB2, forms large ring complexes (prohibitin complexes) in the inner mitochondrial membrane (IMM) and functions as chaperone protein that stabilizes mitochondrial respiratory enzymes and maintains mitochondrial integrity in the IMM, which is required for mitochondrial morphogenesis, neuronal survival, and normal lifespan (Probable). The prohibitin complex, with DNAJC19, regulates cardiolipin remodeling and the protein turnover of OMA1 in a cardiolipin-binding manner. Regulates mitochondrial respiration activity playing a role in cellular aging. The prohibitin complex plays a role of mitophagy receptor involved in targeting mitochondria for autophagic degradation. Involved in mitochondrial-mediated antiviral innate immunity, activates RIG-I-mediated signal transduction and production of IFNB1 and pro-inflammatory cytokine IL6. In the nucleus, acts as a transcription coregulator, enhances promoter binding by TP53, a transcription factor it activates, but reduces the promoter binding by E2F1, a transcription factor it represses. Interacts with STAT3 to affect IL17 secretion in T-helper Th17 cells. In the plasma membrane, cooperates with CD86 to mediate CD86-signaling in B lymphocytes that regulates the level of IgG1 produced through the activation of distal signaling intermediates. Upon CD40 engagement, required to activate NF-kappa-B signaling pathway via phospholipase C and protein kinase C activation.
Protein Name Prohibitin 1
Database Links Reactome: R-HSA-5673000
Reactome: R-HSA-6802946
Reactome: R-HSA-6802955
Reactome: R-HSA-8949664
Reactome: R-HSA-9649948
Cellular Localisation Mitochondrion Inner Membrane
Nucleus
Cytoplasm
Cell Membrane
Alternative Antibody Names Anti-Prohibitin 1 antibody
Anti-PHB1 antibody
Anti-PHB antibody

Information sourced from Uniprot.org

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