Anti-PGK1 antibody (N-Term) (STJA0010315)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human N-ternal PGK1
General Information
| Short Description | Rabbit polyclonal anti-Phosphoglycerate kinase 1 (N-Term) for use in IHC and WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000IHC 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PGK1 |
| Gene ID | 5230 |
| Uniprot ID | PGK1_HUMAN |
| Immunogen | Synthesized peptide derived from human N-ternal PGK1 |
| Immunogen Region | N-Term |
| Specificity | This antibody detects endogenous levels of PGK1 at Human, Mouse, Rat |
Additional Info
| Post Translational Modifications | Phosphorylated at Ser-203 by MAPK1/ERK2 under hypoxic conditions, which promotes its mitochondrial targeting. |
| Function | Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. Both L- and D- forms of purine and pyrimidine nucleotides can be used as substrates, but the activity is much lower on pyrimidines. In addition to its role as a glycolytic enzyme, it seems that PGK1 acts as a polymerase alpha cofactor protein (primer recognition protein). Acts as a protein kinase when localized to the mitochondrion where it phosphorylates pyruvate dehydrogenase kinase PDK1 to inhibit pyruvate dehydrogenase complex activity and suppress the formation of acetyl-coenzyme A from pyruvate, and consequently inhibit oxidative phosphorylation and promote glycolysis. May play a role in sperm motility. |
| Protein Name | Phosphoglycerate Kinase 1Cell Migration-Inducing Gene 10 ProteinPrimer Recognition Protein 2Prp 2 |
| Database Links | Reactome: R-HSA-70171Reactome: R-HSA-70263Reactome: R-HSA-9636667 |
| Cellular Localisation | CytoplasmCytosolMitochondrion MatrixHypoxic Conditions Promote Mitochondrial TargetingTargeted To The Mitochondrion Following Phosphorylation By Mapk1/Erk2Cis-Trans Isomerization By Pin1And Binding To Mitochondrial Circrna Mcpgk1 |
| Alternative Antibody Names | Anti-Phosphoglycerate Kinase 1 antibodyAnti-Cell Migration-Inducing Gene 10 Protein antibodyAnti-Primer Recognition Protein 2 antibodyAnti-Prp 2 antibodyAnti-PGK1 antibodyAnti-PGKA antibodyAnti-MIG10 antibodyAnti-OK antibodyAnti-SW-cl.110 antibody |
Information sourced from Uniprot.org