• Immunohistochemistry analysis of paraffin-embedded mouse kidney using PGK1 Antibody (STJ115974) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human esophagus using PGK1 Antibody (STJ115974) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon using PGK1 Antibody (STJ115974) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat intestine using PGK1 Antibody (STJ115974) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using PGK1 antibody (STJ115974) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-PGK1 antibody (1-417) (STJ115974)

SKU:
STJ115974

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PGK1 (1-417) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PGK1
Gene ID: 5230
Uniprot ID: PGK1_HUMAN
Immunogen Region: 1-417
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-417 of human PGK1 (NP_000282.1).
Immunogen Sequence: MSLSNKLTLDKLDVKGKRVV MRVDFNVPMKNNQITNNQRI KAAVPSIKFCLDNGAKSVVL MSHLGRPDGVPMPDKYSLEP VAVELKSLLGKDVLFLKDCV GPEVEKACANPAAGSVILLE NLRFHVEEEGKGKDASGNKV KAEPAKIEAFRASLSKLGDV YVNDAFGTAHRAHSSMVGVN LPQKAGGFLMKKELNYFAKA LESPERPFLAILGGAKVADK IQLINNMLDKVNEMIIGGG
Tissue Specificity Mainly expressed in spermatogonia. Localized on the principle piece in the sperm (at protein level). Expression significantly decreased in the testis of elderly men.
Function Catalyzes one of the two ATP producing reactions in the glycolytic pathway via the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein). May play a role in sperm motility.
Protein Name Phosphoglycerate Kinase 1
Cell Migration-Inducing Gene 10 Protein
Primer Recognition Protein 2
Prp 2
Database Links Reactome: R-HSA-70171
Reactome: R-HSA-70263
Reactome: R-HSA-9636667
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Phosphoglycerate Kinase 1 antibody
Anti-Cell Migration-Inducing Gene 10 Protein antibody
Anti-Primer Recognition Protein 2 antibody
Anti-Prp 2 antibody
Anti-PGK1 antibody
Anti-PGKA antibody
Anti-MIG10 antibody
Anti-OK antibody
Anti-SW-cl.110 antibody

Information sourced from Uniprot.org

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