• Western blot analysis of various lysates using PFN2 Rabbit polyclonal antibody (STJ11104403) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PFN2 antibody (1-140) (STJ11104403)

SKU:
STJ11104403

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Profilin-2 (1-140) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PFN2
Gene ID: 5217
Uniprot ID: PROF2_HUMAN
Immunogen Region: 1-140
Immunogen: Recombinant Protein corresponding to a sequence within amino acids 1-140 of human PFN2 (NP_444252.1).
Immunogen Sequence: MAGWQSYVDNLMCDGCCQEA AIVGYCDAKYVWAATAGGVF QSITPIEIDMIVGKDREGFF TNGLTLGAKKCSVIRDSLYV DGDCTMDIRTKSQGGEPTYN VAVGRAGRVLVFVMGKEGVH GGGLNKKAYSMAKYLRDSGF
Tissue Specificity Highly expressed in brain, skeletal muscle and kidney and less strongly in heart, placenta, lung and liver.
Function Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG.
Protein Name Profilin-2
Profilin Ii
Database Links Reactome: R-HSA-376176
Reactome: R-HSA-5663220
Cellular Localisation Cytoplasm
Cytoskeleton
Alternative Antibody Names Anti-Profilin-2 antibody
Anti-Profilin Ii antibody
Anti-PFN2 antibody

Information sourced from Uniprot.org

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