Anti-PFKL antibody [R04-7A4] (STJA0034713)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide of human PFKL
General Information
| Short Description | Rabbit monoclonal anti-PFKL for use in WB, ICC and IF in Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ICC/IF |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R04-7A4 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.3 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | WB 1:500-1:1000IF 1:50-1:200 |
| Formulation | 50mM Tris-Glycine (pH 7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | PFKL |
| Gene ID | 5211 |
| Uniprot ID | PFKAL_HUMAN |
| Immunogen | A synthetic peptide of human PFKL |
Additional Info
| Post Translational Modifications | GlcNAcylation at Ser-529 by OGT decreases enzyme activity, leading to redirect glucose flux through the oxidative pentose phosphate pathway. Glycosylation is stimulated by both hypoxia and glucose deprivation. |
| Function | Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis. Negatively regulates the phagocyte oxidative burst in response to bacterial infection by controlling cellular NADPH biosynthesis and NADPH oxidase-derived reactive oxygen species. Upon macrophage activation, drives the metabolic switch toward glycolysis, thus preventing glucose turnover that produces NADPH via pentose phosphate pathway. |
| Protein Name | Atp-Dependent 6-Phosphofructokinase - Liver TypeAtp-PfkPfk-L6-Phosphofructokinase Type BPhosphofructo-1-Kinase Isozyme BPfk-BPhosphohexokinase |
| Database Links | Reactome: R-HSA-6798695Reactome: R-HSA-70171 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Names | Anti-Atp-Dependent 6-Phosphofructokinase - Liver Type antibodyAnti-Atp-Pfk antibodyAnti-Pfk-L antibodyAnti-6-Phosphofructokinase Type B antibodyAnti-Phosphofructo-1-Kinase Isozyme B antibodyAnti-Pfk-B antibodyAnti-Phosphohexokinase antibodyAnti-PFKL antibody |
Information sourced from Uniprot.org