Western blot analysis of various lysates using PFKFB2 Rabbit pAb (STJ111650) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-PFKFB2 antibody (446-505) (STJ111650)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ111650
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General Information

Short DescriptionRabbit polyclonal PFKFB2 (446-505) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IF/ICC/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolPFKFB2
Gene ID5208
Uniprot IDF262_HUMAN
Immunogen Region446-505
Immunogen SequenceRDKPTNNFPKNQTPVRMRRN SFTPLSSSNTIRRPRNYSVG SRPLKPLSPLRAQDMQEGAD
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 446-505 of human PFKFB2 (NP_006203.2).

Additional Info

Tissue Specificity Heart.
Post Translational Modifications Phosphorylation by AMPK stimulates activity.
Function Synthesis and degradation of fructose 2,6-bisphosphate.
Protein Name 6-Phosphofructo-2-Kinase/Fructose-2 -6-Bisphosphatase 2
6pf-2-K/Fru-2 -6-P2ase 2
Pfk/Fbpase 2
6pf-2-K/Fru-2 -6-P2ase Heart-Type Isozyme Includes - 6-Phosphofructo-2-Kinase - Fructose-2 -6-Bisphosphatase
Database Links Reactome: R-HSA-9634600
Cellular Localisation
Alternative Antibody Names Anti-6-Phosphofructo-2-Kinase/Fructose-2 -6-Bisphosphatase 2 antibody
Anti-6pf-2-K/Fru-2 -6-P2ase 2 antibody
Anti-Pfk/Fbpase 2 antibody
Anti-6pf-2-K/Fru-2 -6-P2ase Heart-Type Isozyme Includes - 6-Phosphofructo-2-Kinase - Fructose-2 -6-Bisphosphatase antibody
Anti-PFKFB2 antibody

Information sourced from Uniprot.org

Citations

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