Anti-PEX2/PXMP3 antibody (Internal) (STJ72263)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-PEX2/PXMP3 (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Applications | Pep-ELISA/WB |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Dog/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 35kDa band observed in lysates of cell line HeLa (calculated MW of 34.9kDa according to NP_000309.1). Recommended concentration: 1-3µg/ml. |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | PEX2 |
| Gene ID | 5828 |
| Uniprot ID | PEX2_HUMAN |
| Accession Number | NP_000309.1 |
| Immunogen Region | Internal |
| Immunogen Sequence | NATVGQSVLNIKYKN |
| Specificity | Reported variants represent identical protein: NP_001073336.1, NP_000309.1 |
Additional Info
| Function | E3 ubiquitin-protein ligase component of a retrotranslocation channel required for peroxisome organization by mediating export of the PEX5 receptor from peroxisomes to the cytosol, thereby promoting PEX5 recycling. The retrotranslocation channel is composed of PEX2, PEX10 and PEX12.each subunit contributing transmembrane segments that coassemble into an open channel that specifically allows the passage of PEX5 through the peroxisomal membrane. PEX2 also regulates peroxisome organization by acting as a E3 ubiquitin-protein ligase. PEX2 ubiquitinates PEX5 during its passage through the retrotranslocation channel: catalyzes monoubiquitination of PEX5 at 'Cys-11', a modification that acts as a signal for PEX5 extraction into the cytosol. Required for pexophagy in response to starvation by mediating ubiquitination of peroxisomal proteins, such as PEX5 and ABCD3/PMP70. Also involved in the response to reactive oxygen species (ROS) by mediating 'Lys-48'-linked polyubiquitination and subsequent degradation of PNPLA2/ATGL, thereby regulating lipolysis. |
| Protein Name | Peroxisome Biogenesis Factor 235 Kda Peroxisomal Membrane ProteinPeroxin-2Peroxisomal Membrane Protein 3Peroxisome Assembly Factor 1Paf-1Ring Finger Protein 72 |
| Database Links | Reactome: R-HSA-8866654Reactome: R-HSA-9033241Reactome: R-HSA-9603798 |
| Cellular Localisation | Peroxisome MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Peroxisome Biogenesis Factor 2 antibodyAnti-35 Kda Peroxisomal Membrane Protein antibodyAnti-Peroxin-2 antibodyAnti-Peroxisomal Membrane Protein 3 antibodyAnti-Peroxisome Assembly Factor 1 antibodyAnti-Paf-1 antibodyAnti-Ring Finger Protein 72 antibodyAnti-PEX2 antibodyAnti-PAF1 antibodyAnti-PMP3 antibodyAnti-PMP35 antibodyAnti-PXMP3 antibodyAnti-RNF72 antibody |
Information sourced from Uniprot.org