Anti-Perforin antibody (413-552) [ZR270] (STJ180619)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenRecombinant human Perforin protein fragment (around aa 413-552)
General Information
| Short Description | Rabbit monoclonal Perforin (413-552) antibody for use in IHC-P in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC-P |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | ZR270 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity purified |
| Dilution Range | 1:100-200 |
| Formulation | Tris-HCI buffer containing stabilizing protein (BSA) and <0.1% ProClin |
| Storage Instruction | Store at 2‐8°C for up to 24 months. Predilute: Ready to use, no reconstitution necessary. Concentrate: Use dilution range and appropriate lab‐standardized diluent. Stability after dilution: 7 days at 24°C, 3 months at 2‐8°C, 6months at ‐20°C. |
Target Information
| Gene Symbol | PRF1 |
| Gene ID | 5551 |
| Uniprot ID | PERF_HUMAN |
| Immunogen | Recombinant human Perforin protein fragment (around aa 413-552) |
| Immunogen Region | 413-552 |
| Specificity | Positive Control: Spleen |
Additional Info
| Post Translational Modifications | N-glycosylated. |
| Function | Pore-forming protein that plays a key role in granzyme-mediated programmed cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by mediating the passage and uptake of cytotoxic granzymes. Facilitates the delivery of cationic cargo protein, while anionic or neural proteins are not delivered efficiently. Perforin pores allow the release of mature caspase-7 (CASP7) into the extracellular milieu. |
| Protein Name | Perforin-1P1CytolysinLymphocyte Pore-Forming ProteinPfp |
| Database Links | Reactome: R-HSA-9725371 |
| Cellular Localisation | Cytolytic GranuleSecretedCell MembraneMulti-Pass Membrane ProteinEndosome LumenStored In Cytolytic Granules Of Cytolytic T-Lymphocytes And Secreted Into The Cleft Between T-Lymphocyte And Target CellInserts Into The Cell Membrane Of Target Cells And Forms PoresMembrane Insertion And Pore Formation Requires A Major Conformation ChangeMay Be Taken Up Via Endocytosis Involving Clathrin-Coated Vesicles And Accumulate In A First Time In Large Early Endosomes |
| Alternative Antibody Names | Anti-Perforin-1 antibodyAnti-P1 antibodyAnti-Cytolysin antibodyAnti-Lymphocyte Pore-Forming Protein antibodyAnti-Pfp antibodyAnti-PRF1 antibodyAnti-PFP antibody |
Information sourced from Uniprot.org