• Immunohistochemical analysis of paraffin-embedded human-spleen, antibody was diluted at 1:200
  • Immunohistochemical analysis of paraffin-embedded human-spleen, antibody was diluted at 1:200
  • Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200
  • Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200
  • Western blot analysis of Hela cells using Perforin 1 Polyclonal Antibody diluted at 1:500. Secondary antibody was diluted at 1:20000

Anti-Perforin 1 antibody (STJ98623)

SKU:
STJ98623

Current Stock:
Host: Rabbit
Applications: WB, IHC, ELISA
Reactivity: Human
Short Description: Rabbit polyclonal anti-Perforin 1 antibody is suitable for use in Western Blot, Immunohistochemistry and ELISA.
Note: FOR RESEARCH USE ONLY (RUO).
Clonality: Polyclonal
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using a epitope-specific immunogen.
Storage Instruction: Store at-20°C, and avoid repeat freeze-thaw cycles.
Concentration: 1 mg/ml
Isotype: IgG
Dilution Range: WB 1:500-2000
IHC 1:50-200
ELISA 1:10000-20000
Specificity: Perforin 1 polyclonal antibody detects endogenous levels of Perforin 1
Uniprot ID: PERF_HUMAN
Gene ID: 5551
Gene Symbol: PRF1
Immunogen: Synthesized peptide derived from Perforin 1
Immunogen Region: 451-500 aa
Function Pore-forming protein that plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.
Protein Name Perforin-1
P1
Cytolysin
Lymphocyte Pore-Forming Protein
Pfp
Cellular Localisation Cytoplasmic Granule Lumen
Secreted
Cell Membrane
Multi-Pass Membrane Protein
Endosome Lumen
Stored In Cytoplasmic Granules Of Cytolytic T-Lymphocytes And Secreted Into The Cleft Between T-Lymphocyte And Target Cell
Inserts Into The Cell Membrane Of Target Cells And Forms Pores
Membrane Insertion And Pore Formation Requires A Major Conformation Change
May Be Taken Up Via Endocytosis Involving Clathrin-Coated Vesicles And Accumulate In A First Time In Large Early Endosomes
Alternative Antibody Names Anti-Perforin-1 antibody
Anti-P1 antibody
Anti-Cytolysin antibody
Anti-Lymphocyte Pore-Forming Protein antibody
Anti-Pfp antibody
Anti-PRF1 antibody
Anti-PFP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance