• Western blot analysis of extracts of various cell lines, using PELP1 rabbit monoclonal antibody (STJ11102411) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of extracts of Rat testis, using PELP1 Rabbit monoclonal antibody (STJ11102411) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of Rat testis, using PELP1 rabbit monoclonal antibody (STJ11102411) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using PELP1 Rabbit monoclonal antibody (STJ11102411) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PELP1 antibody (1000-1100) [S1MR] (STJ11102411)

SKU:
STJ11102411

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-PELP1 (1000-1100) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PELP1
Gene ID: 27043
Uniprot ID: PELP1_HUMAN
Immunogen Region: 1000-1100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1000-1100 of human PELP1 (Q8IZL8).
Immunogen Sequence: EPEPGLLLEVEEPGTEEERG ADTAPTLAPEALPSQGEVER EGESPAAGPPPQELVEEEPS APPTLLEEETEDGSDKVQPP PETPAEEEMETETEAEALQE K
Tissue Specificity Widely expressed.
Post Translational Modifications Transiently sumoylated, preferentially conjugated to SUMO2 or SUMO3. Sumoylation causes nucleolar exclusion of PELP1 and promotes the recruitment of MDN1 to pre-60S particles. Desumoylation by SUMO isopeptidase SENP3 is needed to release both PELP1 and MDN1 from pre-ribosomes.
Function Coactivator of estrogen receptor-mediated transcription and a corepressor of other nuclear hormone receptors and sequence-specific transcription factors. Plays a role in estrogen receptor (ER) genomic activity when present in the nuclear compartment by activating the ER target genes in a hormonal stimulation dependent manner. Can facilitate ER non-genomic signaling via SRC and PI3K interaction in the cytosol. Plays a role in E2-mediated cell cycle progression by interacting with RB1. May have important functional implications in ER/growth factor cross-talk. Interacts with several growth factor signaling components including EGFR and HRS. Functions as the key stabilizing component of the Five Friends of Methylated CHTOP (5FMC) complex.the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes. Component of the PELP1 complex involved in the nucleolar steps of 28S rRNA maturation and the subsequent nucleoplasmic transit of the pre-60S ribosomal subunit. Regulates pre-60S association of the critical remodeling factor MDN1. May promote tumorigenesis via its interaction with and modulation of several oncogenes including SRC, PI3K, STAT3 and EGFR. Plays a role in cancer cell metastasis via its ability to modulate E2-mediated cytoskeleton changes and cell migration via its interaction with SRC and PI3K.
Protein Name Proline- - Glutamic Acid- And Leucine-Rich Protein 1
Modulator Of Non-Genomic Activity Of Estrogen Receptor
Transcription Factor Hmx3
Database Links Reactome: R-HSA-6791226
Reactome: R-HSA-8849473
Cellular Localisation Nucleus
Nucleolus
Nucleoplasm
Cytoplasm
Mainly Found In The Nucleoplasm
With Low Levels Detected In The Cytoplasm
Also Found Associated With The Plasma Membrane
Mainly In Cytoplasm In A Subset Of Breast Tumors
Localization Is Widely Deregulated In Endometrial Cancers With Predominantly Cytoplasm Localization In High-Grade Endometrial Tumors
Alternative Antibody Names Anti-Proline- - Glutamic Acid- And Leucine-Rich Protein 1 antibody
Anti-Modulator Of Non-Genomic Activity Of Estrogen Receptor antibody
Anti-Transcription Factor Hmx3 antibody
Anti-PELP1 antibody
Anti-HMX3 antibody
Anti-MNAR antibody

Information sourced from Uniprot.org

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